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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Performance of immunohistochemistry as a useful tool for the diagnosis of cutaneous leishmaniasis in Panama, Central America

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Author(s):
Gonzalez, K. [1, 2] ; Calzada, J. E. [2] ; Diaz, R. [3, 4] ; Paz, H. [2] ; Garcia, V. [3, 4] ; Miranda, A. [2] ; Tomokane, T. [1] ; Puga, S. [2] ; Saldana, A. [2] ; Laurenti, M. [1]
Total Authors: 10
Affiliation:
[1] Univ Sao Paulo, Fac Med, Lab Patol Molestias Infecciosas, Sao Paulo, SP - Brazil
[2] ICGES, Panama City - Panama
[3] Univ Sao Paulo, Fac Med, Sao Paulo, SP - Brazil
[4] Hosp Santo Tomas, Lab Patol, Panama City - Panama
Total Affiliations: 4
Document type: Journal article
Source: Parasitology International; v. 71, p. 46-52, AUG 2019.
Web of Science Citations: 2
Abstract

Cutaneous leishmaniasis (CL) is one of the most frequent parasitic zoonoses in Panama. Currently, conventional, molecular and histopathological tests are performed to diagnose CL. Immunohistochemistry (IHC) has proven to be a valuable tool to facilitate the diagnosis of leishmaniasis and to study the cellular immune response developed during the infection. Therefore, considering the absence of IHC in the diagnostic routine in Panama, the objective of this study is to demonstrate the usefulness of this test as a complementary diagnostic tool for improving the sensitivity of histopathology (HP) and helping to study the cellular immune response of patients. Samples from patients with suspected CL were analysed by intradermal reaction of Montenegro (IDRM), smears, culture, PCR (Viannia, Hsp-70), HP and IHC. According to the diagnostic criteria, 95.8% of patients were positive for Leishmania sp., that was characterized as Leishmania (V.) panarnensis by PCR-HSP7O/RFLP. From positive samples, Leishmania was detected by the tested diagnostic methods in the following degrees: 100% by IDRM, 60% by smears, 93.3% by culture, 100% by kDNA PCR, 78.3% by PCR Hsp-70, 50% by HP and 73.9% by IHC. Although IHC had a poor correlation (k = 0.191) with the diagnostic criteria, the sensitivities of both HP (76.1%) and smears (89.1%) were improved by combining them with IHC. IHC considerably improved the detection of the Leishmania parasites in the histopathological sections, supporting the need to implement this diagnostic tool in Panama. In addition, immunohistochemistry allows evaluation of the patient's immune response and thus provides new guidelines for the treatment and control of CL in Panama. (AU)

FAPESP's process: 14/50315-0 - Leishmaniasis in Latin America: an advanced perspective on immunopathogenetic factors of cutaneous and visceral infection, immunomodulators of the sandflies vector saliva and immunogenic exo-antigens of Leishmania (L.) infantum chagasi as vaccine candidates
Grantee:Marcia Dalastra Laurenti
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 17/03141-5 - Characterization of histopathological changes and cellular immune response in cutaneous lesions of patients with Tegumentary Leishmaniasis in Panamá
Grantee:Kadir Amilcar Gonzalez Carrion
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)