Variation in DNA methylation in the KvDMR1 (ICR2) region in first-trimester human pregnancies

Author(s):Show less -

Miranda Furtado, Cristiana Libardi ^{[1, 2, 3]} ; Salomao, Karina Bezerra ^[2] ; Verruma, Carolina Gennari ^[2] ; Paulino Leite, Sarah Blima ^[2] ; Lopes Rios, Alvaro Fabricio ^[4] ; Bialecka, Monika ^[1] ; Moustakas, Ioannis ^{[1, 5]} ; Mei, Hailiang ^[5] ; Paro de Paz, Claudia Cristina ^{[2, 6]} ; Duarte, Geraldo ^[3] ; Lopes, Susana M. Chuva de Sousa ^[1] ; Ramos, Ester Silveira ^[2]

Affiliation

^[1] Leiden Univ, Med Ctr, Dept Anat & Embryol, Leiden - Netherlands

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Gynecol & Obstet, Sao Paulo - Brazil

^[4] State Univ North Fluminense Darcy Ribeiro, Ctr Biosci & Biotechnol, Biotechnol Lab, Campos Dos Goytacazes, RJ - Brazil

^[5] Leiden Univ, Med Ctr, Dept Biomed Data Sci, Sequencing Anal Support Core, Leiden - Netherlands

^[6] Ctr APTA Bovinos Corte, Inst Zootecnia, Sao Paulo - Brazil

• Document type: Journal article
• Source: Fertility and Sterility; v. 111, n. 6, p. 1186-1193, JUN 2019.
• Web of Science Citations: 1

Abstract

Objective: To investigate the levels of DNA methylation in the KvDMR1 (KvLQT1 differentially methylated region 1) in embryonic and extra-embryonic tissues. Design: Cross-sectional study. Setting: University medical center and clinical hospital. Patient(s): Embryonic and/or extraembryonic tissues (umbilical cord, chorionic villus, chorion, decidua, and/or amnion) collected from 27 first-trimester pregnancies (up to 12 weeks of gestation, single embryos) from elective abortions, extravillous trophoblasts (EVTs) from the top of individual chorionic villi, and chorionic villi from 10 normal full-term placentas collected after birth. Intervention(s): None. Main Outcome Measure(s): DNA methylation of the KvDMR1 region evaluated using quantitative analysis of DNA methylation followed by real-time polymerase chain reaction (qAMP) and bisulfite sequencing (bis-seq) analysis. Result(s): The results showed variability in KvDMR1 DNA methylation in different tissues from the same pregnancy. The average of DNA methylation was not different between the embryo, umbilical cord, amnion, and chorionic villi, despite the relatively low level of methylation observed in the amnion (33.50% +/- 14.48%). Chorionic villi from term placentas showed a normal methylation pattern at KvDMR1 (42.60% +/- 6.08%). The normal methylation pattern at KvDMR1 in chorionic villi (as well as in EVTs) from first-trimester placentas was confirmed by bis-seq. Conclusion(s): Our results highlight an existing heterogeneity in DNA methylation of the KvDMR1 region during first trimester and a consistent hypomethylation in the amnion in this period of gestation. (Copyright (C) 2019 American Society for Reproductive Medicine, Published by Elsevier Inc.) (AU)

• FAPESP's process: 12/11069-9 - ANDROGEN RECEPTOR CAG POLIMORFISM AND TELOMERE LENGTH IN PREMATURE OVARIAN FAILURE
• Grantee: Cristiana Libardi Miranda Furtado
• Support Opportunities: Scholarships in Brazil - Post-Doctoral