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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Oral streptococci show diversity in resistance to complement immunity

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Author(s):
Alves, Livia A. [1] ; de Carli, Thais R. [1] ; Harth-Chu, Erika N. [1] ; Mariano, Flavia S. [1] ; Hofling, Jose F. [1] ; Stipp, Rafael N. [1] ; Mattos-Graner, Renata O. [1]
Total Authors: 7
Affiliation:
[1] Univ Estadual Campinas, Dept Oral Diag, Piracicaba Dent Sch, Piracicaba, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Journal of Medical Microbiology; v. 68, n. 4, p. 600-608, APR 2019.
Web of Science Citations: 2
Abstract

Purpose. Mechanisms underlying systemic infections by oral species of Mitis (Streptococcus mitis, Streptococcus oralis) and Sanguinis (Streptococcus gordonii, Streptococcus sanguinis) commensal streptococci are poorly understood. This study investigates profiles of susceptibility to complement-mediated host immunity in representative strains of these four species, which were isolated from oral sites or from the bloodstream. Methodology. Deposition of complement opsonins (C3b/iC3b), and surface binding to C-reactive protein (CRP) and to IgG antibodies were quantified by flow cytometry in 34 strains treated with human serum (HS), and compared to rates of opsonophagocytosis by human PMN mediated by complement (CR1/3) and/or IgG Fc (Fc gamma RII/III) receptors. Results. S. sanguinis strains showed reduced susceptibility to complement opsonization and low binding to CRP and to IgG compared to other species. Surface levels of C3b/iC3b in S. sanguinis strains were 4.5-and 7.8-fold lower than that observed in S. gordonii and Mitis strains, respectively. Diversity in C3b/iC3b deposition was evident among Mitis species, in which C3b/iC3b deposition was significantly associated with CR/Fc gamma R-dependent opsonophagocytosis by PMN (P<0.05). Importantly, S. gordonii and Mitis group strains isolated from systemic infections showed resistance to complement opsonization when compared to oral isolates of the respective species (P<0.05). Conclusions. This study establishes species-specific profiles of susceptibility to complement immunity in Mitis and Sanguinis streptococci, and indicates that strains associated with systemic infections have increased capacity to evade complement immunity. These findings highlight the need for studies identifying molecular functions involved in complement evasion in oral streptococci. (AU)

FAPESP's process: 18/02054-4 - Identification of factors modulating the susceptibility of Streptococcus sanguinis to complement immunity
Grantee:Renata de Oliveira Mattos Graner
Support Opportunities: Regular Research Grants
FAPESP's process: 13/07098-6 - Evaluation of the susceptibility of oral Streptococcus ssp. to the marking by the complement system
Grantee:Thaís Rodrigues de Carli
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 15/12940-3 - Identification of surface proteins of Streptococcus mutans involved in evasion of opsonization by the complement system
Grantee:Renata de Oliveira Mattos Graner
Support Opportunities: Regular Research Grants
FAPESP's process: 09/50547-0 - Characterization of GbpB/PcsB homologues in commensal species of oral streptococci
Grantee:Erika Nikitza Shiauha Harth Chu
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 12/04222-5 - Analysis of the roles of the transcriptional regulators VicRK and CovR in the susceptibility of Streptococcus mutans to opsonization by the complement system.
Grantee:Lívia Araújo Alves
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 12/50966-6 - Analysis of the roles of the transchptional regulators VicRK and CovR in the susceptibility of Streptococcus mutans and Streptococcus sanguinis to opsonization by the complement system
Grantee:Renata de Oliveira Mattos Graner
Support Opportunities: Regular Research Grants
FAPESP's process: 15/07237-1 - Identification of surface proteins of Streptococcus mutans involved in the scape to opsonization by the complement system
Grantee:Lívia Araújo Alves
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 17/19899-4 - Identification of factors modulating the susceptibility of Streptococcus sanguinis to complement immunity
Grantee:Lívia Araújo Alves
Support Opportunities: Scholarships in Brazil - Post-Doctoral