| Full text | |
| Author(s): |
Bachiega, Patricia
[1]
;
de Almeida, Eduardo
[2]
;
Salgado, Jocelem Mastrodi
[1]
;
Zezzi Arruda, Marco Aurelio
[3, 4]
;
Lehmann, Eraldo Luiz
[3, 4]
;
Morzelle, Maressa Caldeira
[5]
;
Pereira de Carvalho, Hudson Wallace
[2]
Total Authors: 7
|
| Affiliation: | [1] Univ Sao Paulo, Esalq, Escola Super Agr Luiz de Queiroz, Dept Agroind Alimentos & Nutr, Ave Padua Dias 111, BR-13418900 Piracicaba, SP - Brazil
[2] Univ Sao Paulo, CENA, LIN, BR-13416000 Piracicaba - Brazil
[3] Univ Estadual Campinas, UNICAMP, GEPAM, Dept Quim Analit, Inst Quim, POB 6154, BR-13083970 Campinas, SP - Brazil
[4] Univ Estadual Campinas, UNICAMP, Inst Quim, Inst Nacl Ciencia & Tecnol Bioanalit, BR-13083970 Campinas, SP - Brazil
[5] Univ Fed Mato Grosso, FANUT, UFMT, Dept Alimentos Nutr, Fac Nutr, Rua Fernando Correa Costa 2367, BR-78060900 Cuiaba, MT - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | FOOD ANALYTICAL METHODS; v. 12, n. 7, p. 1520-1527, JUL 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
Biofortification of crops with selenium (Se) is an effective strategy to increase the consumption of this micronutrient. Inductively coupled plasma mass spectrometry (ICP-MS) is one of the most used techniques in quantifying Se. However, due to some disadvantages (high cost, long analysis time, and being destructive), there is a need for new alternatives to Se quantification. This study aimed at establishing instrumental parameters for Se quantification, using two energy-dispersive X-ray fluorescence spectrometry (EDXRF) techniques (benchtop and handheld) in Se-biofortified broccoli seedlings and comparing it with ICP-MS. The results showed that the selection of proper filters for both EDXRF systems was crucial for determining Se and, when compared with ICP-MS, presented similar results for selenium-biofortified broccoli seedling treatments (BS50Se). In addition, the EDXRF techniques presented a Se limit of detection (LOD) at 0.6-0.9mgkg(-1). This study demonstrates that EDXRF systems were successfully applied to concentration measurement of Se in biofortified samples and linked to low cost and shorter analysis time. (AU) | |
| FAPESP's process: | 15/05942-0 - Shedding light on the metabolism of nanomaterials absorbed by plants with X-ray and vibrational spectroscopy |
| Grantee: | Hudson Wallace Pereira de Carvalho |
| Support Opportunities: | Research Grants - Young Investigators Grants |