Shedding light on the metabolism of nanomaterials absorbed by plants with X-ray and vibrational spectroscopy

• Grant number: 15/05942-0
• Support Opportunities: Research Grants - Young Investigators Grants
• Duration: August 01, 2015 - July 31, 2019
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Hudson Wallace Pereira de Carvalho
• Grantee: Hudson Wallace Pereira de Carvalho
• Host Institution: Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated researchers: Adriana Pinheiro Martinelli ; Bruno Leonardo Caetano ; Francisco José Krug
• Associated grant(s): 16/50014-6 - Tailoring oxide based nanoparticles for the mineral nutrition of plants, AP.R SPRINT; 15/19121-8 - Multi-User Equipment approved in grant 2015/05942-0: micro X-Ray Fluorescence system, AP.EMU
• Associated scholarship(s): 18/13401-7 - X-ray spectroscopy unfolding the effects of glyphosate on soybean manganese foliar uptake, BP.MS; 18/02462-5 - Alignment, coding and commissioning of a total reflection X ray fluorescence (TXRF) system under vaccum and automatic for agricultural and environmental research, BP.MS; 17/16375-4 - Effects of foliar application of La and Ce on soybean, BP.MS; 16/08589-1 - Evaluation of nanoparticles agglomeration and quality control of nutrient solution of plants in hydroponic cultivation, BP.IC; 16/04611-2 - Evaluation of root absorption and displacement of ZnO nanoparticles in Phaseolus vulgaris (beans), BP.MS

Abstract

The development of goods containing nanomaterials has been rising in the last years. On the other hand, the ecotoxicological implications, assimilation and reactivity of nanomaterials within living beings are not well understood. Furthermore such research subjects are still incipient worldwide. In this context the present proposal aims at creating a research group and acquire de necessary infrastructure needed to study the absorption, transport, accumulation and chemical transformations of nanomaterials (ZnO, ZrO2 and CuO) incorporated by plants (Phaseolus vulgaris, i.e., Brazilian bean). The absorbed nanomaterials and metallic micro nutrients will be quantified and the parameters that influence the uptake of nanomaterials such as effect of particle size, concentration and time of exposure will be evaluated in root and foliar systems. Plant organs will be scanned by micro X-ray fluorescence locating the specialised tissues of storage. The distribution of organic metabolites around the accumulation spots will be mapped by Raman scattering spectroscopy. The combination of these two techniques will result in chemical images which will contribute to the understanding of the metabolism in the surroundings of the accumulation spots. In addition, plant detoxification mechanisms will be revealed through the investigation of the chemical environment of the incorporated nanomaterials by X-ray absorption spectroscopy. Finally, the kinetics of transport and storage will be explored by in vivo micro X-ray fluorescence. (AU)

Scientific publications

(41)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERESIN GOMES, MARCOS HENRIQUE; MIGLIAVACCA, RAFAELA ALENBRANT; OTTO, RAFAEL; PEREIRA DE CARVALHO, HUDSON WALLACE. Physicochemical characterization of fertilizers containing concentrated suspensions of CuO, MnCO3 and ZnO. Scientia Agricola, v. 77, n. 6, 2020. (15/05942-0)

DURAN, NADIA M.; MEDINA-LLAMAS, MARIA; CASSANJI, JOAO G. B.; DE LIMA, RAFAEL G.; DE ALMEIDA, EDUARDO; MACEDO, WILLIAN R.; MATTIA, DAVIDE; PEREIRA DE CARVALHO, HUDSON W.. Bean Seedling Growth Enhancement Using Magnetite Nanoparticles. Journal of Agricultural and Food Chemistry, v. 66, n. 23, p. 5746-5755, JUN 13 2018. (15/19121-8, 15/05942-0)

SAVASSA, SUSILAINE MAIRA; CASTILLO-MICHEL, HIRAM; PRADAS DEL REAL, ANA ELENA; REYES-HERRERA, JUAN; MARQUES, JOAO PAULO RODRIGUES; DE CARVALHO, HUDSON W. P.. Ag nanoparticles enhancing Phaseolus vulgaris seedling development: understanding nanoparticle migration and chemical transformation across the seed coat. ENVIRONMENTAL SCIENCE-NANO, v. 8, n. 2, p. 493-501, FEB 1 2021. (15/19121-8, 15/05942-0)

KOTLAR, ALI MEHMANDOOST; PEREIRA DE CARVALHO, HUDSON WALLACE; IVERSEN, BO V.; VAN LIER, QUIRIJN DE JONG. Nitrate leaching from layered double hydroxides in tropical and temperate soils. APPLIED CLAY SCIENCE, v. 184, JAN 2020. (15/05942-0, 16/18636-7)

DA CRUZ, TATIANA N. M.; SAVASSA, SUSILAINE M.; GOMES, MARCOS H. F.; RODRIGUES, EDUARDO S.; DURAN, NADIA M.; DE ALMEIDA, EDUARDO; MARTINELLI, ADRIANA P.; DE CARVALHO, HUDSON W. P.. Shedding light on the mechanisms of absorption and transport of ZnO nanoparticles by plants via in vivo X-ray spectroscopy. ENVIRONMENTAL SCIENCE-NANO, v. 4, n. 12, p. 2367-2376, DEC 2017. (15/19121-8, 16/04611-2, 15/05942-0, 16/08589-1)

LANZA, MARIA GABRIELA DANTAS BERETA; SILVA, VINICIUS MARTINS; MONTANHA, GABRIEL SGARBIERO; LAVRES, JOSE; DE CARVALHO, HUDSON WALLACE PEREIRA; DOS REIS, ANDRE RODRIGUES. Assessment of selenium spatial distribution using mu-XFR in cowpea (Vigna unguiculata (L.) Walp.) plants: Integration of physiological and biochemical responses. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 207, JAN 1 2021. (15/26817-9, 18/15490-7, 15/19121-8, 15/05942-0)

RODRIGUES, EDUARDO S.; MONTANHA, GABRIEL S.; DE ALMEIDA, EDUARDO; FANTUCCI, HUGO; SANTOS, RAFAEL M.; DE CARVALHO, HUDSON W. P.. Effect of nano cerium oxide on soybean (Glycine max L. Merrill) crop exposed to environmentally relevant concentrations. Chemosphere, v. 273, JUN 2021. (15/19121-8, 15/05942-0, 17/16375-4)

MONTANHA, GABRIEL SGARBIERO; RODRIGUES, EDUARDO SANTOS; MARQUES, JOAO PAULO RODRIGUES; DE ALMEIDA, EDUARDO; DOS REIS, ANDRE RODRIGUES; PEREIRA DE CARVALHO, HUDSON WALLACE. X-ray fluorescence spectroscopy (XRF) applied to plant science: challenges towards in vivo analysis of plants. METALLOMICS, v. 12, n. 2, p. 183-192, FEB 1 2020. (15/05942-0, 17/16375-4, 15/19121-8)

LAU, ELIZABETH C. H. T.; CARVALHO, LUCAS B.; PEREIRA, ANDERSON E. S.; MONTANHA, GABRIEL S.; CORREA, CAMILA G.; CARVALHO, HUDSON W. P.; GANIN, ALEXEY Y.; FRACETO, LEONARDO F.; YIU, HUMPHREY H. P.. Localization of Coated Iron Oxide (Fe3O4) Nanoparticles on Tomato Seeds and Their Effects on Growth. ACS APPLIED BIO MATERIALS, v. 3, n. 7, p. 4109-4117, JUL 20 2020. (18/02413-4, 18/23608-8, 15/19121-8, 15/05942-0)

RODRIGUES, EDUARDO S.; MONTANHA, GABRIEL S.; MARQUES, JOAO P. R.; DE ALMEIDA, EDUARDO; YABUKI, LAUREN N. M.; MENEGARIO, AMAURI A.; PEREIRA DE CARVALHO, HUDSON W.. Foliar application of rare earth elements on soybean (Glycine max (L)): Effects on biometrics and characterization of phytotoxicity. JOURNAL OF RARE EARTHS, v. 38, n. 10, p. 1131-1139, OCT 2020. (17/16375-4, 15/05942-0)

GOMES, MARCOS H. F.; MACHADO, BIANCA A.; RODRIGUES, EDUARDO S.; MONTANHA, GABRIEL SGARBIERO; ROSSI, MONICA LANZONI; OTTO, RAFAEL; LINHARES, FRANCISCO S.; CARVALHO, HUDSON W. P.. In Vivo Evaluation of Zn Foliar Uptake and Transport in Soybean Using X-ray Absorption and Fluorescence Spectroscopy. Journal of Agricultural and Food Chemistry, v. 67, n. 44, p. 12172-12181, NOV 6 2019. (18/13401-7, 15/05942-0)

DE ALMEIDA, EDUARDO; DURAN, NADIA M.; GOMES, MARCOS H. F.; SAVASSA, SUSILAINE M.; DA CRUZ, TATIANA N. M.; MIGLIAVACCA, RAFAELA A.; PEREIRA DE CARVALHO, HUDSON W.. EDXRF for elemental determination of nanoparticle-related agricultural samples. X-RAY SPECTROMETRY, v. 48, n. 2, p. 94-104, MAR-APR 2019. (15/05942-0)

MACHADO, BIANCA DE ALMEIDA; FERESIN GOMES, MARCOS HENRIQUE; DE ALMEIDA, EDUARDO; OTTO, RAFAEL; KAMOGAWA, MARCOS YASSUO; PEREIRA DE CARVALHO, HUDSON WALLACE. Understanding the chemistry of manganese fertilizers and glyphosate mixtures by using synchrotron X-ray spectrometry. SN APPLIED SCIENCES, v. 2, n. 11, p. 12-pg., 2020-10-17. (15/05942-0, 18/13401-7)

GOMES, MARCOS HENRIQUE FERESIN; DE ALMEIDA MACHADO, BIANCA; MARQUES, JOAO PAULO RODRIGUES; OTTO, RAFAEL; EICHERT, THOMAS; PEREIRA DE CARVALHO, HUDSON WALLACE. Foliar Application of Zn Phosphite and Zn EDTA in Soybean (Glycine max(L.) Merrill): In Vivo Investigations of Transport, Chemical Speciation, and Leaf Surface Changes. JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION, v. 20, n. 4, p. 9-pg., 2020-09-16. (15/05942-0, 18/13401-7, 15/19121-8)

FERESIN GOMES, MARCOS HENRIQUE; MIGLIAVACCA, RAFAELA ALENBRANT; OTTO, RAFAEL; PEREIRA DE CARVALHO, HUDSON WALLACE. Physicochemical characterization of fertilizers containing concentrated suspensions of CuO, MnCO3 and ZnO. CIENTIA AGRICOL, v. 77, n. 6, p. 6-pg., 2020-01-01. (15/05942-0)

GOMES, MARCOS HENRIQUE FERESIN; DE ALMEIDA MACHADO, BIANCA; MARQUES, JOAO PAULO RODRIGUES; OTTO, RAFAEL; EICHERT, THOMAS; PEREIRA DE CARVALHO, HUDSON WALLACE. Foliar Application of Zn Phosphite and Zn EDTA in Soybean (Glycine max(L.) Merrill): In Vivo Investigations of Transport, Chemical Speciation, and Leaf Surface Changes. JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION, v. 20, n. 4, p. 2731-2739, DEC 2020. (15/19121-8, 18/13401-7, 15/05942-0)

DURAN, NADIA M.; SAVASSA, SUSILAINE M.; DE LIMA, RAFAEL GIOVANINI; DE ALMEIDA, EDUARDO; LINHARES, FRANCISCO S.; VAN GESTEL, CORNELIS A. M.; PEREIRA DE CARVALHO, HUDSON W.. X-ray Spectroscopy Uncovering the Effects of Cu Based Nanoparticle Concentration and Structure on Phaseolus vulgaris Germination and Seedling Development. Journal of Agricultural and Food Chemistry, v. 65, n. 36, p. 7874-7884, SEP 13 2017. (15/19121-8, 15/05942-0)

SANTINI, LUIZ; ORTEGA RODRIGUEZ, DAIGARD RICARDO; QUINTILHAN, MANOLO TRINDADE; BRAZOLIN, SERGIO; TOMMASIELLO FILHO, MARIO. Evidence to wood biodeterioration of tropical species revealed by non-destructive techniques. Science of The Total Environment, v. 672, p. 357-369, JUL 1 2019. (15/05942-0, 15/19121-8, 14/17312-8)

MONTANHA, GABRIEL SGARBIERO; RODRIGUES, EDUARDO SANTOS; RODRIGUES MARQUES, JOAO PAULO; DE ALMEIDA, EDUARDO; COLZATO, MARINA; PEREIRA DE CARVALHO, HUDSON WALLACE. Zinc nanocoated seeds: an alternative to boost soybean seed germination and seedling development. SN APPLIED SCIENCES, v. 2, n. 5, MAY 2020. (15/05942-0, 15/19121-8)

DE ALMEIDA, EDUARDO; MELQUIADES, FABIO L.; MARQUES, JOAO P. R.; MARGUI, EVA; DE CARVALHO, HUDSON W. P.. Determination of the polymeric thin film thickness by energy dispersive X-ray fluorescence and multivariate analysis. SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY, v. 167, MAY 2020. (15/05942-0)

RODRIGUES MARQUES, JOAO PAULO; SPOSITO, MARCEL BELLATO; AMORIM, LILIAN; MONTANHA, GABRIEL SGARBIERO; SILVA JUNIOR, GERALDO JOSE; PEREIRA DE CARVALHO, HUDSON WALLACE; APPEZZATO-DA-GLORIA, BEATRIZ. Persistent Calyxes in Postbloom Fruit Drop: A Microscopy and Microanalysis Perspective. PATHOGENS, v. 9, n. 4, APR 2020. (15/05942-0, 08/54176-4, 15/19121-8)

MARUCCI, ROSANGELA CRISTINA; FREITAS, LARAH MARTINS; SANTOS-RASERA, JOYCE RIBEIRO; ALVES, DEJANE SANTOS; CARVALHO, GERALDO ANDRADE; PEREIRA DE CARVALHO, HUDSON WALLACE. Are cerium oxide nanoparticles transferred from plants to the aphid Myzus perskae (Hemiptera: Aphididae)?. FLORIDA ENTOMOLOGIST, v. 102, n. 3, p. 555-561, SEP 2019. (15/05942-0, 15/19121-8)

RODRIGUES MARQUES, JOAO PAULO; AFERRI, GABRIELA; MONTANHA, GABRIEL SGABIEIRO; PERASSOLO GUEDES, FERNANDA TRILSTZ; SOARES, MARLI MISAKI; MUNIZ, LARISSA FERNANDO; TOMAZELLO FILHO, MARIO; XAVIER, MAURO ALEXANDRE; PEREIRA DE CARVALHO, HUDSON WALLACE. ugarcane as a Forage Plant: Structural and Chemical Traits that Affect Fiber Qualit. BIORESOURCES, v. 16, n. 4, p. 7622-7633, NOV 2021. (18/18851-0, 15/19121-8, 15/05942-0)

SANTOS-RASERA, JOYCE RIBEIRO; SANT'ANNA NETO, ANALDER; ROSIM MONTEIRO, REGINA TERESA; VAN GESTEL, CORNELIS A. M.; PEREIRA DE CARVALHO, HUDSON WALLACE. Toxicity, bioaccumulation and biotransformation of Cu oxide nanoparticles in Daphnia magna. ENVIRONMENTAL SCIENCE-NANO, v. 6, n. 9, p. 2897-2906, SEP 1 2019. (15/05942-0)

DA CRUZ, TATIANA N. M.; SAVASSA, SUSILAINE M.; MONTANHA, GABRIEL S.; ISHIDA, JULIANE K.; DE ALMEIDA, EDUARDO; TSAI, SIU M.; LAVRES JUNIOR, JOSE; PEREIRA DE CARVALHO, HUDSON W.. A new glance on root-to-shoot in vivo zinc transport and time-dependent physiological effects of ZnSO4 and ZnO nanoparticles on plants. SCIENTIFIC REPORTS, v. 9, JUL 18 2019. (15/05942-0, 16/04611-2)

BACHIEGA, PATRICIA; DE ALMEIDA, EDUARDO; SALGADO, JOCELEM MASTRODI; ZEZZI ARRUDA, MARCO AURELIO; LEHMANN, ERALDO LUIZ; MORZELLE, MARESSA CALDEIRA; PEREIRA DE CARVALHO, HUDSON WALLACE. Benchtop and Handheld Energy-Dispersive X-Ray Fluorescence (EDXRF) as Alternative for Selenium Concentration Measurement in Biofortified Broccoli Seedling. FOOD ANALYTICAL METHODS, v. 12, n. 7, p. 1520-1527, JUL 2019. (15/05942-0)

SAVASSA, SUSILAINE M.; DURAN, NADIA M.; RODRIGUES, EDUARDO S.; DE ALMEIDA, EDUARDO; VAN GESTEL, CORNELIS A. M.; BOMPADRE, THIAGO F. V.; DE CARVALHO, HUDSON W. P.. Effects of ZnO Nanoparticles on Phaseolus vulgaris Germination and Seedling Development Determined by X-ray Spectroscopy. ACS APPLIED NANO MATERIALS, v. 1, n. 11, p. 6414-6426, NOV 2018. (15/19121-8, 15/05942-0)

CASTRO, BARBARA M. M.; SANTOS-RASERA, JOYCE R.; ALVES, DEJANE S.; MARUCCI, ROSANGELA C.; CARVALHO, GERALDO A.; CARVALHO, HUDSON W. P.. Ingestion and effects of cerium oxide nanoparticles on Spodoptera frugiperda (Lepidoptera: Noctuidae). Environmental Pollution, v. 279, JUN 15 2021. (15/05942-0, 15/19121-8)

ANDRADE, DANIEL FERNANDES; DE ALMEIDA, EDUARDO; PEREIRA DE CARVALHO, HUDSON WALLACE; PEREIRA-FILHO, EDENIR RODRIGUES; AMARASIRIWARDENA, DULASIRI. Chemical inspection and elemental analysis of electronic waste using data fusion - Application of complementary spectroanalytical techniques. Talanta, v. 225, APR 1 2021. (16/01513-0, 15/05942-0, 16/17304-0, 18/24569-6, 19/24223-5)

MENDES, NANDHARA ANGELICA CARVALHO; LANZA, MARIA GABRIELA DANTAS BERETA; SILVA, VINICIUS MARTINS; BOSSE, MARCO ANTONIO; MONTANHA, GABRIEL SGARBIERO; DE CARVALHO, HUDSON WALLACE PEREIRA; CUNHA, MATHEUS LUIS OLIVEIRA; DOS REIS, ANDRE RODRIGUES. Selenium Increases Ureide Metabolism and Yield of Cowpea Plants: Examining Se Distribution in Leaves and Seeds Using Microprobe X-Ray Fluorescence Spectroscopy. GESUNDE PFLANZEN, v. 75, n. 5, p. 12-pg., 2023-03-08. (18/10912-0, 15/19121-8, 15/05942-0)

SANTOS-RASERA, JOYCE RIBEIRO; ROSIM MONTEIRO, REGINA TERESA; PEREIRA DE CARVALHO, HUDSON WALLACE. Investigation of acute toxicity, accumulation, and depuration of ZnO nanoparticles in Daphnia magna. Science of The Total Environment, v. 821, p. 8-pg., 2022-01-29. (15/05942-0)

ORTEGA RODRIGUEZ, DAIGARD RICARDO; HEVIA, ANDREA; SANCHEZ-SALGUERO, RAUL; SANTINI, LUIZ; PEREIRA DE CARVALHO, HUDSON WALLACE; ROIG, FIDEL A.; TOMAZELLO-FILHO, MARIO. Exploring wood anatomy, density and chemistry profiles to understand the tree-ring formation in Amazonian tree species. DENDROCHRONOLOGIA, v. 71, p. 17-pg., 2022-02-01. (15/05942-0, 09/53951-7, 15/19121-8, 17/50085-3, 18/22914-8, 19/05675-2, 19/07576-1)

MACHADO, BIANCA A.; GOMES, MARCOS H. F.; MARQUES, JOAO P. R.; OTTO, RAFAEL; DE CARVALHO, HUDSON W. P.. X-ray Spectroscopy Fostering the Understanding of Foliar Uptake and Transport of Mn by Soybean (Glycine max L. Merril): Kinetics, Chemical Speciation, and Effects of Glyphosate. Journal of Agricultural and Food Chemistry, v. 67, n. 47, p. 13010-13020, NOV 27 2019. (18/13401-7, 15/05942-0, 15/19121-8)

ORTEGA RODRIGUEZ, DAIGARD RICARDO; DE ALMEIDA, EDUARDO; TOMAZELLO-FILHO, MARIO; PEREIRA DE CARVALHO, HUDSON WALLACE. Space-resolved determination of the mineral nutrient content in tree-rings by X-ray fluorescence. Science of The Total Environment, v. 708, MAR 15 2020. (15/05942-0, 15/19121-8)

FERESIN GOMES, MARCOS HENRIQUE; CALLAGHAN, CIARAN; SIPANO MENDES, ANTONIO CESAR; EDLER, KAREN J.; MATTIA, DAVIDE; DE JONG VAN LIER, QUIRIJN; PEREIRA DE CARVALHO, HUDSON WALLACE. Cellulose Microbeads: Toward the Controlled Release of Nutrients to Plants. ACS AGRICULTURAL SCIENCE & TECHNOLOGY, v. 2, n. 2, p. 9-pg., 2022-04-18. (18/13401-7, 15/19121-8, 15/05942-0)

SANTOS-RASERA, JOYCE RIBEIRO; DE LIMA, RAFAEL GIOVANINI; ALVES, DEJANE SANTOS; ROSIM MONTEIRO, REGINA TERESA; PEREIRA DE CARVALHO, HUDSON WALLACE. X-ray spectrometry imaging and chemical speciation assisting to understand the toxic effects of copper oxide nanoparticles on zebrafish (Danio rerio). Nanotoxicology, v. 16, n. 5, p. 13-pg., 2022-10-18. (15/05942-0)

DOS REIS, ANDRE RODRIGUES; MARCANDALLI BOLETA, EDUARDO HENRIQUE; ALVES, CHARLINE ZARATIN; COTRIM, MAYARA FAVERO; BARBOSA, JULIERME ZIMMER; SILVA, VINICIUS MARTINS; PORTO, RAFAEL LAWANDOVSKI; DANTAS BERETA LANZA, MARIA GABRIELA; LAVRES, JOSE; FERESIN GOMES, MARCOS HENRIQUE; et al. Selenium toxicity in upland field-grown rice: Seed physiology responses and nutrient distribution using the mu-XRF technique. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, v. 190, MAR 1 2020. (15/05942-0, 16/19937-0, 15/19121-8)

MONTANHA, GABRIEL S.; RODRIGUES, EDUARDO S.; ROMEU, SARA L. Z.; DE ALMEIDA, EDUARDO; REIS, ANDRE R.; LAVRES JR, JOSE; PEREIRA DE CARVALHO, HUDSON W.. Zinc uptake from ZnSO4 (aq) and Zn-EDTA ((aq)) and its root-to-shoot transport in soybean plants (Glycine max) probed by time-resolved in vivo X-ray spectroscopy. Plant Science, v. 292, MAR 2020. (15/19121-8, 15/05942-0, 17/16375-4)

ORTEGA RODRIGUEZ, DAIGARD RICARDO; PEREIRA DE CARVALHO, HUDSON WALLACE; TOMAZELLO-FILHO, MARIO. Nutrient concentrations of 17-year-old Pinus taeda annual tree-rings analyzed by X-ray fluorescence microanalysis. DENDROCHRONOLOGIA, v. 52, p. 67-79, DEC 2018. (15/19121-8, 15/05942-0)

DE OLIVEIRA, JESSICA BEZERRA; RODRIGUES MARQUES, JOAO PAULO; RODAK, BRUNA WURR; GALINDO, FERNANDO SHINTATE; CARR, NATALIA FERNANDES; ALMEIDA, EDUARDO; ARAKI, KOITI; GONCALVES, JOSUE MARTINS; DOS REIS, ANDRE RODRIGUES; VAN DER ENT, ANTONY; et al. Fate of nickel in soybean seeds dressed with different forms of nickel. RHIZOSPHERE, v. 21, p. 10-pg., 2022-03-01. (15/05942-0, 19/04585-0, 18/13738-1, 15/19121-8)

ALMEIDA, EDUARDO DE; MONTANHA, GABRIEL SGABIERO; CARVALHO, HUDSON W. PEREIRA DE; MARGUI, EVA. Evaluation of energy dispersive X-ray fluorescence and total reflection X-ray fluorescence spectrometry for vegetal mass-limited sample analysis: Application to soybean root and shoots. SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY, v. 170, AUG 2020. (15/05942-0, 15/19121-8)