Surrogate production of Salmo salar oocytes and sperm in triploid Oncorhynchus mykiss by germ cell transplantation technology

Author(s)

Hattori, Ricardo Shohei ^[1] ; Yoshinaga, Tulio Teruo ^[2] ; Katayama, Naoto ^{[3, 4]} ; Hattori-Ihara, Shoko ^[3] ; Tsukamoto, Ricardo Yasuichi ^[5] ; Takahashi, Neuza Sumico ^[6] ; Tabata, Yara Aiko ^[1]

Affiliation

^[1] Sao Paulo Fisheries Inst APTA SAA, Caixa Postal 361, BR-12460000 Campos Do Jordao, SP - Brazil

^[2] Univ Sao Paulo, Dept Surg, Sch Vet Med & Anim Sci, BR-05508070 Sao Paulo - Brazil

^[3] Tokyo Univ Marine Sci & Technol, Dept Marine Biosci, Tokyo 1088477 - Japan

^[4] Nippon Suisan Kaisha Ltd, Tokyo 1920991 - Japan

^[5] Puriaqua Ltd, BR-01237010 Sao Paulo - Brazil

^[6] Sao Paulo Fisheries Inst APTA SAA, BR-05001900 Sao Paulo - Brazil

• Document type: Journal article
• Source: Aquaculture; v. 506, p. 238-245, MAY 15 2019.
• Web of Science Citations: 0

Abstract

Surrogate broodstock technology is emerging as a promising biotechnology for xenogeneic production of gametes of highly valuable fish species. In this study, we produced gametes of landlocked Atlantic salmon using triploid rainbow trout as recipient through germ cell transplantation technique in embryos. Spermatogonial cells were obtained from Atlantic salmon juveniles and transplantation conducted into the coelomic cavity of 150 mixed-sex triploid embryos of rainbow trout. Colonization efficiency assessed in larvae four weeks post-transplantation showed presence of PKH-26-labeled cells in 61.1% of individuals. Analysis in sexually mature adults revealed presence of donor-derived sperm in four transplanted males (10%), whereby one of them reached sexual maturity in the first year. Transplanted females became mature after the second year and donor-derived oocytes were detected in four individuals (12.1%). Milt produced by transplanted triploid males were used for artificial insemination. Genetic analysis of hatchlings revealed that progeny was composed by pure Atlantic salmon and therefore were donor-derived. In conclusion, this study shows that surrogate broodstock can be used to produce oocytes and spermatozoa using species from different genus. The production of surrogate gametes using species with a shorter life cycle represents an efficient approach to streamline the development of improved strains in aquaculture industry. (AU)

• FAPESP's process: 13/17612-9 - Genetic characterization, sex control, and germ cell transplantation in salmonids stocks from Estação Experimental de Salmonicultura de Campos do Jordão
• Grantee: Ricardo Shohei Hattori
• Support Opportunities: Research Grants - Young Investigators Grants