A systems biology view of wood formation in Eucalyptus grandis trees submitted to different potassium and water regimes

Author(s):Show less -

Ployet, Raphael ^[1] ; Veneziano Labate, Monica T. ^[2] ; Cataldi, Thais Regiani ^[2] ; Christina, Mathias ^{[3, 4]} ; Morel, Marie ^[1] ; San Clemente, Helene ^[1] ; Denis, Marie ^{[5, 6]} ; Favreau, Benedicte ^{[5, 6]} ; Tomazello Filho, Mario ^[3] ; Laclau, Jean-Paul ^{[3, 4]} ; Labate, Carlos Alberto ^[2] ; Chaix, Gilles ^{[3, 5, 6]} ; Grima-Pettenati, Jacqueline ^[1] ; Mounet, Fabien ^[1]

Affiliation

^[1] Univ Toulouse III, Lab Rech Sci Vegetates, CNRS, UPS, F-31326 Castanet Tolosan - France

^[2] Univ Sao Paulo, Max Faffer Lab Plant Genet, Dept Genet, Coll Agr Luiz de Queiroz, Av Padua Dias 11, POB 09, BR-13418900 Piracicaba, SP - Brazil

^[3] Univ Sao Paulo, Dept Forest Resource, Luiz de Queiroz Coll Agr, Av Padua Dias 11, BR-13418900 Sao Paulo - Brazil

^[4] CIRAD, UMR ECO & SOLS, F-34398 Montpellier - France

^[5] CIRAD, UMR AGAP, F-34395 Montpellier 9 - France

^[6] Montpellier SupAgro, INRA, CIRAD, UMR AGAP, Montpellier - France

• Document type: Journal article
• Source: NEW PHYTOLOGIST; v. 223, n. 2, p. 766-782, JUL 2019.
• Web of Science Citations: 2

Abstract

Wood production in fast-growing Eucalyptus grandis trees is highly dependent on both potassium (K) fertilization and water availability but the molecular processes underlying wood formation in response to the combined effects of these two limiting factors remain unknown. E. grandis trees were submitted to four combinations of K-fertilization and water supply. Weighted gene co-expression network analysis and MixOmics-based co-regulation networks were used to integrate xylem transcriptome, metabolome and complex wood traits. Functional characterization of a candidate gene was performed in transgenic E. grandis hairy roots. This integrated network-based approach enabled us to identify meaningful biological processes and regulators impacted by K-fertilization and/or water limitation. It revealed that modules of co-regulated genes and metabolites strongly correlated to wood complex traits are in the heart of a complex trade-off between biomass production and stress responses. Nested in these modules, potential new cell-wall regulators were identified, as further confirmed by the functional characterization of EgMYB137. These findings provide new insights into the regulatory mechanisms of wood formation under stressful conditions, pointing out both known and new regulators co-opted by K-fertilization and/or water limitation that may potentially promote adaptive wood traits. (AU)

• FAPESP's process: 13/25642-5 - Water and mineral stress effects on cambial activities and wood formation in eucalyptus grandis: identification of key properties for genotype*environment interaction studies under global change
• Grantee: Mario Tommasiello Filho
• Support type: Research Program on Global Climate Change - Regular Grants