Genome-wide sequencing and metabolic annotation of Pythium irregulare CBS 494.86: understanding Eicosapentaenoic acid production

Author(s)

Fernandes, Bruna S. ^{[1, 2]} ; Dias, Oscar ^[1] ; Costa, Gisela ^[1] ; Kaupert Neto, Antonio A. ^[3] ; Resende, Tiago F. C. ^[1] ; Oliveira, Juliana V. C. ^[3] ; Riano-Pachon, Diego M. ^[4] ; Zaiat, Marcelo ^[5] ; Pradella, Jose G. C. ^[6] ; Rocha, Isabel ^[1]

Affiliation

^[1] Univ Minho, Ctr Biol Engn, Braga - Portugal

^[2] Univ Fed Pernambuco, Dept Civil & Environm Engn, Recife, PE - Brazil

^[3] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Bioethanol Sci & Technol Lab CTBE, Campinas, SP - Brazil

^[4] Univ Sao Paulo, Computat Evolutionary & Syst Biol Lab, Ctr Nucl Energy Agr, Piracicaba, SP - Brazil

^[5] Univ Sao Paulo, Sao Carlos Sch Engn EESC, Ctr Res Dev & Innovat Environm Engn, Biol Proc Lab, Sao Carlos, SP - Brazil

^[6] PRBiotec Ltd, Sao Jose Dos Campos, SP - Brazil

• Document type: Journal article
• Source: BMC Biotechnology; v. 19, JUN 28 2019.
• Web of Science Citations: 0

Abstract

BackgroundPythium irregulare is an oleaginous Oomycete able to accumulate large amounts of lipids, including Eicosapentaenoic acid (EPA). EPA is an important and expensive dietary supplement with a promising and very competitive market, which is dependent on fish-oil extraction. This has prompted several research groups to study biotechnological routes to obtain specific fatty acids rather than a mixture of various lipids. Moreover, microorganisms can use low cost carbon sources for lipid production, thus reducing production costs. Previous studies have highlighted the production of EPA by P. irregulare, exploiting diverse low cost carbon sources that are produced in large amounts, such as vinasse, glycerol, and food wastewater. However, there is still a lack of knowledge about its biosynthetic pathways, because no functional annotation of any Pythium sp. exists yet. The goal of this work was to identify key genes and pathways related to EPA biosynthesis, in P. irregulare CBS 494.86, by sequencing and performing an unprecedented annotation of its genome, considering the possibility of using wastewater as a carbon source.ResultsGenome sequencing provided 17,727 candidate genes, with 3809 of them associated with enzyme code and 945 with membrane transporter proteins. The functional annotation was compared with curated information of oleaginous organisms, understanding amino acids and fatty acids production, and consumption of carbon and nitrogen sources, present in the wastewater. The main features include the presence of genes related to the consumption of several sugars and candidate genes of unsaturated fatty acids production.ConclusionsThe whole metabolic genome presented, which is an unprecedented reconstruction of P. irregulare CBS 494.86, shows its potential to produce value-added products, in special EPA, for food and pharmaceutical industries, moreover it infers metabolic capabilities of the microorganism by incorporating information obtained from literature and genomic data, supplying information of great importance to future work. (AU)

• FAPESP's process: 16/10562-4 - Evaluation of lipid production in silico, eicosapentaenoic acid (EPA), by pythium irregular as value added product, from vinasse
• Grantee: Bruna Soares Fernandes
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor