| Full text | |
| Author(s): |
Steiner, Marcelo Luis
[1]
;
Theodoro, Therese Rachell
[2]
;
Garcia, Shirley Gimenez
[3]
;
Amaral Antonio Mader, Ana Maria
[4]
;
Pompei, Luciano de Melo
[1]
;
da Silva Pinhal, Maria Aparecida
[2]
;
Fernandes, Cesar Eduardo
[1]
Total Authors: 7
|
| Affiliation: | [1] Fac Med ABC, Dept Gynecol & Obstet, Santo Andre, SP - Brazil
[2] Fac Med ABC, Dept Biochem, Santo Andre, SP - Brazil
[3] Fac Med ABC, Santo Andre, SP - Brazil
[4] Fac Med ABC, Dept Pathol, Santo Andre, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Revista Brasileira de Ginecologia e Obstetrícia; v. 41, n. 7, p. 449-453, JUL 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
Abstract Objective To analyze the effects of estrogen alone or in combination with progestogens and tibolone (TIB) on the expression of the extracellular matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9), of perlecan, and of heparanase (HPSE) of the vascular walls of the carotid arteries. Methods A total of 30 250-day-old ovariectomized Wistar rats were orally treated for 5 weeks with: a) 1 mg/kg of estradiol benzoate (EB); b) EB + 0.2 mg/kg of medroxyprogesterone acetate (MPA); c) EB + 0.2mg/kg of norethisterone acetate (NETA); d) EB + 2 mg/kg of dydrogesterone (DI); e) 1 mg/kg of TIB; f) placebo (CTR). Following treatment, the expression of mRNA for MMP-2, MMP-9, and HPSE was analyzed by realtime polymerase chain-reaction (PCR), and the expression of MMP-2, of MMP-9, of tissue inhibitor of metalloproteinase 2 (TIMP-2), and of perlecan was quantified by immunohistochemistry in the carotid arteries. Results The groups showed significant differences on mRNA HPSE expression (p = 0.048), which was higher in the EB, EB + MPA, and TIB groups. There was no statistically significant difference in mRNA MMP-2 or MMP-9 expression. The immunohistochemical expression of MMP-2, of TIMP-2, of MMP-9, of HPSE, and of perlecan showed no differences between groups. Conclusion Estradiol alone or associated with MPA and TIB treatment can increase mRNA HSPE expression of the walls of the carotid arteries in ovariectomized rats. (AU) | |
| FAPESP's process: | 11/13704-0 - Effect of tibolone, estradiol and estradiol combined with medroxyprogesterone acetate or norethindrone acetate or micronized progesterone in the presence of vascular matrix metalloproteinases and histomorphometric changes in breast tissue of rats |
| Grantee: | Luciano de Melo Pompei |
| Support Opportunities: | Regular Research Grants |