Amino acid restriction increases beta-cell death under challenging conditions

Author(s)

Goncalves, Luciana Mateus ^[1] ; Vettorazzi, Jean Franciesco ^[1] ; Vanzela, Emerielle Cristine ^[1] ; Figueiredo, Mariana Sarto ^[1] ; Batista, Thiago Martins ^[1] ; Zoppi, Claudio Cesar ^[1] ; Boschero, Antonio Carlos ^[1] ; Carneiro, Everardo Magalhaes ^[1]

Affiliation

^[1] Univ Estadual Campinas, Dept Struct & Funct Biol, OCRC, Inst Biol, Campinas, SP - Brazil

• Document type: Journal article
• Source: Journal of Cellular Physiology; v. 234, n. 10, p. 16679-16684, OCT 2019.
• Web of Science Citations: 0

Abstract

Malnutrition programs metabolism, favor dysfunction of beta cells. We aimed to establish an in vitro protocol of malnutrition, assessing the effect of amino acid restriction upon the beta cells. Insulin-producing cells INS-1E and pancreatic islets were maintained in RPMI 1640 medium containing 1x (Ctl) or 0.25x (AaR) of amino acids. We evaluated several markers of beta-cell function and viability. AaR Insulin secretion was reduced, whereas cell viability was unaltered. Calcium oscillations in response to glucose increased in AaR. AaR showed lower Ins1 RNAm, snap 25, and PKC (protein kinase C) protein content, whereas phospho-eIF2 alpha was increased. AaR cells exposed to nutrient or chemical challenges displayed higher apoptosis rates. We showed that amino acid restriction programmed beta cell and induced functional changes. This model might be useful for the study of molecular mechanisms involved with beta-cell programming helping to establish novel therapeutic targets to prevent harmful outcomes of malnutrition. (AU)

• FAPESP's process: 14/01717-9 - Investigation of the insulinotropic, insulinomimetic and endothelial actions of taurine in cells/tissues submitted to an in vitro amino acid restriction: an integrated and multifocal approach
• Grantee: Everardo Magalhães Carneiro
• Support Opportunities: Research Projects - Thematic Grants