| Full text | |
| Author(s): |
Fuzita, Felipe J.
[1]
;
Pimenta, Daniel C.
[2]
;
Palmisano, Giuseppe
[3]
;
Terra, Walter R.
[1]
;
Ferreira, Clelia
[1]
Total Authors: 5
|
| Affiliation: | [1] Univ Sao Paulo, Inst Quim, Dept Bioquim, Ave Prof Lineu Prestes 748, BR-05508000 Sao Paulo - Brazil
[2] Inst Butantan, Lab Bioquim & Biofis, Ave Vital Brasil 1500, BR-05503900 Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-05508000 Sao Paulo - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY; v. 235, p. 8-18, SEP 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
The midgut from lepidopteran insects has a particular way to release proteins to the lumen, named microapocrine secretion that could be an adaptation to release secretory contents into the lumen at water absorbing regions. In this process small vesicles (microapocrine vesicles) bud from the midgut microvilli as double membrane vesicles, where the inner membrane comes from the secretion vesicle and the outer one from the microvillar membrane. The molecular machinery associated with this process may be recruited by specific midgut microvilli membrane domains. To address to this, Spodoptera frugiperda midgut microvillar membranes, prepared by magnesium treatment and free from cytoskeleton with the hyperosmotic Tris procedure, were submitted to detergent extraction and fractionated by density gradient ultracentrifugation. Detergent-resistant membrane domains (DRM) were recovered and their proteins identified by proteomics. Microapocrine vesicles were isolated by washing the lumina] surface of the midgut epithelium, followed by freezing and thawing plus centrifugation to recover only membranes. Proteins from purified microvillar membranes and microapocrine vesicle membranes were identified by proteomics. Comparison of the two populations suggests that the budding of microapocrine vesicles surrounded by microvillar membrane is not a random process, because only around 50% of the microvillar membrane proteins are in the microapocrine vesicles. From the 16 proteins from DRM, 14 were enriched in the microapocrine membrane vesicles. These results suggest that on budding, the microapocrine vesicle membrane is enclosed by DRM and a surrounding area of the microvillar membrane. It is proposed that the DRMs somehow recruit the proteins composing the secretory machinery. (AU) | |
| FAPESP's process: | 17/08103-4 - Insect digestion: a molecular, cellular, physiological and evolutionary approach |
| Grantee: | Walter Ribeiro Terra |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 14/14183-2 - Intestinal microvillar membranes and their secretory vesicles in Lepidoptera Spodoptera frugiperda and Diptera Musca domestica |
| Grantee: | Felipe Jun Fuzita |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 14/06863-3 - Post-translational modifications in cancer and parasite infection diagnosis: methodological approaches and biological implications |
| Grantee: | Giuseppe Palmisano |
| Support Opportunities: | Research Grants - Young Investigators Grants |