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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Kinin B-1 Receptor Acts in Adipose Tissue to Control Fat Distribution in a Cell-Nonautonomous Manner

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Author(s):
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Sales, Vicencia M. [1] ; Goncalves-Zillo, Thais [1] ; Castoldi, Angela [2] ; Burgos, Marina [2] ; Branquinho, Jessica [1] ; Batista, Carolina [1] ; Oliveira, Valeria [1] ; Silva, Elton [1] ; Castro, Charlles H. M. [3] ; Camara, Niels [2] ; Mori, Marcelo A. [1, 4] ; Pesquero, Joao Bosco [1]
Total Authors: 12
Affiliation:
[1] Univ Fed Sao Paulo UNIFESP, Dept Biophys, Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Immunol, Sao Paulo - Brazil
[3] Univ Fed Sao Paulo UNIFESP, Dept Med, Sao Paulo - Brazil
[4] Univ Estadual Campinas UNICAMP, Dept Biochem & Tissue Biol, Campinas, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Diabetes; v. 68, n. 8, p. 1614-1623, AUG 2019.
Web of Science Citations: 1
Abstract

The kinin B-1 receptor (B1R) plays a role in inflammatory and metabolic processes. B1R deletion (B-1(-/-)) protects mice from diet-induced obesity and improves insulin and leptin sensitivity. In contrast, genetic reconstitution of B1R exclusively in adipose tissue reverses the lean phenotype of B-1(-/-) mice. To study the cell-nonautonomous nature of these effects, we transplanted epididymal white adipose tissue (eWAT) from wild-type donors (B-1(+/+)) into B-1(-/-) mice (B-1(+/+)-> B-1(-/-)) and compared them with autologous controls (B-1(+/+)-> B-1(+/+) or B-1(-/-)-> B-1(-/-)). We then fed these mice a high-fat diet for 16 weeks and investigated their metabolic phenotypes. B-1(+/+)-> B-1(-/-) mice became obese but not glucose intolerant or insulin resistant, unlike B-1(-/-)-> B-1(-/-) mice. Moreover, the endogenous adipose tissue of B-1(+/+)-> B-1(-/-) mice exhibited higher expression of adipocyte markers (e.g., Fabp4 and Adipoq) and changes in the immune cell pool. These mice also developed fatty liver. Wild-type eWAT transplanted into B-1(-/-) mice normalized circulating insulin, leptin, and epidermal growth factor levels. In conclusion, we demonstrated that B1R in adipose tissue controls the response to diet-induced obesity by promoting adipose tissue expansion and hepatic lipid accumulation in cell-nonautonomous manners. (AU)

FAPESP's process: 17/07975-8 - Exploring the role of adipose tissue-derived circulating miRNAs in the beneficial effects of exercise training
Grantee:Marcelo Alves da Silva Mori
Support type: Regular Research Grants
FAPESP's process: 14/27198-8 - Establishment of a center of genetic and molecular research for clinical challenges
Grantee:João Bosco Pesquero
Support type: Research Projects - Thematic Grants
FAPESP's process: 17/01184-9 - CAMeLEOm: cross-species analysis of metabolic, lifespan effects and omics of dietary restriction mimetics
Grantee:Marcelo Alves da Silva Mori
Support type: Research Projects - Thematic Grants