| Full text | |
| Author(s): Show less - |
Sales, Vicencia M.
[1]
;
Goncalves-Zillo, Thais
[1]
;
Castoldi, Angela
[2]
;
Burgos, Marina
[2]
;
Branquinho, Jessica
[1]
;
Batista, Carolina
[1]
;
Oliveira, Valeria
[1]
;
Silva, Elton
[1]
;
Castro, Charlles H. M.
[3]
;
Camara, Niels
[2]
;
Mori, Marcelo A.
[1, 4]
;
Pesquero, Joao Bosco
[1]
Total Authors: 12
|
| Affiliation: | [1] Univ Fed Sao Paulo UNIFESP, Dept Biophys, Sao Paulo - Brazil
[2] Univ Sao Paulo, Dept Immunol, Sao Paulo - Brazil
[3] Univ Fed Sao Paulo UNIFESP, Dept Med, Sao Paulo - Brazil
[4] Univ Estadual Campinas UNICAMP, Dept Biochem & Tissue Biol, Campinas, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Diabetes; v. 68, n. 8, p. 1614-1623, AUG 2019. |
| Web of Science Citations: | 1 |
| Abstract | |
The kinin B-1 receptor (B1R) plays a role in inflammatory and metabolic processes. B1R deletion (B-1(-/-)) protects mice from diet-induced obesity and improves insulin and leptin sensitivity. In contrast, genetic reconstitution of B1R exclusively in adipose tissue reverses the lean phenotype of B-1(-/-) mice. To study the cell-nonautonomous nature of these effects, we transplanted epididymal white adipose tissue (eWAT) from wild-type donors (B-1(+/+)) into B-1(-/-) mice (B-1(+/+)-> B-1(-/-)) and compared them with autologous controls (B-1(+/+)-> B-1(+/+) or B-1(-/-)-> B-1(-/-)). We then fed these mice a high-fat diet for 16 weeks and investigated their metabolic phenotypes. B-1(+/+)-> B-1(-/-) mice became obese but not glucose intolerant or insulin resistant, unlike B-1(-/-)-> B-1(-/-) mice. Moreover, the endogenous adipose tissue of B-1(+/+)-> B-1(-/-) mice exhibited higher expression of adipocyte markers (e.g., Fabp4 and Adipoq) and changes in the immune cell pool. These mice also developed fatty liver. Wild-type eWAT transplanted into B-1(-/-) mice normalized circulating insulin, leptin, and epidermal growth factor levels. In conclusion, we demonstrated that B1R in adipose tissue controls the response to diet-induced obesity by promoting adipose tissue expansion and hepatic lipid accumulation in cell-nonautonomous manners. (AU) | |
| FAPESP's process: | 17/07975-8 - Exploring the role of adipose tissue-derived circulating miRNAs in the beneficial effects of exercise training |
| Grantee: | Marcelo Alves da Silva Mori |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 17/01184-9 - CAMeLEOm: cross-species analysis of metabolic, lifespan effects and omics of dietary restriction mimetics |
| Grantee: | Marcelo Alves da Silva Mori |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 14/27198-8 - Establishment of a center of genetic and molecular research for clinical challenges |
| Grantee: | João Bosco Pesquero |
| Support Opportunities: | Research Projects - Thematic Grants |