Bioproduction of N-acetyl-glucosamine from colloidal alpha-chitin using an enzyme cocktail produced by Aeromonas caviae CHZ306

Author(s)

Cardozo, Flavio Augusto ^[1] ; Facchinatto, William Marcondes ^[2] ; Colnago, Luiz Alberto ^[3] ; Campana-Filho, Sergio Paulo ^[2] ; Pessoa, Adalberto ^[4]

Affiliation

^[1] Univ Sao Paulo, Biomed Sci Inst, Dept Microbiol, 1374 Prof Lineu Prestes Ave, BR-05508000 Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Sao Carlos Inst Chem, Trabalhador Sao Carlense Ave 400, BR-13560970 Sao Carlos, SP - Brazil

^[3] Embrapa Instrumentat, R XV Novembro 1452, BR-13560970 Sao Carlos, SP - Brazil

^[4] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, 580 Prof Lineu Prestes Ave, BR-05508000 Sao Paulo - Brazil

• Document type: Journal article
• Source: WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY; v. 35, n. 8 AUG 2019.
• Web of Science Citations: 0

Abstract

N-acetyl-d-glucosamine (GlcNAc) is an important amino-monosaccharide with great potential for biotechnological applications. It has traditionally been produced by the chemical hydrolysis of chitin, despite certain industrial and environmental drawbacks, including acidic wastes, low yields and high costs. Therefore, enzymatic production has gained attention as a promising environmentally-friendly alternative to the chemical processes. In this study we demonstrate the GlcNAc bioproduction from colloidal alpha-chitin using an enzyme cocktail containing endochitinases and exochitinases (chitobiosidases and N-acetyl-glucosaminidases). The enzyme cocktail was extracted after fermentation in a bioreactor by Aeromonas caviae CHZ306, a chitinolytic marine bacterium with great potential for chitinase production. Hydrolysis parameters were studied in terms of temperature, pH, enzyme and substrate concentration, and reaction time, achieving over 90% GlcNAc yield within 6h. The use of colloidal alpha-chitin as substrate showed a substantial improvement of GlcNAc yields, when compared with beta-chitin and alpha-chitin polymorphs. Such result is directly related to a significant decrease in crystallinity and viscosity from natural alpha-chitin, providing the chitinase with greater accessibility to the depolymerized chains. This study provides valuable information on the GlcNAc bioproduction from chitin using an enzymatic approach, addressing the key points for its production, including the enzyme cocktail composition and the substrate structures. {[}GRAPHICS] . (AU)

• FAPESP's process: 16/20970-2 - Study of chitosan and hydrophilic derivatives by time domain NMR
• Grantee: William Marcondes Facchinatto
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 12/16824-0 - Enzymatic production of N-acetyl-glucosamine by Aeromonas sp. isolated from marine ecosystem
• Grantee: Flávio Augusto Cardozo
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/18773-6 - Chitin convertion to N-acetyl-glucosamine using chitinases of Aeromonas sp. isolated from marine environment
• Grantee: Gabriel Padilla
• Support Opportunities: Regular Research Grants