Alginate-Based Delivery Systems for Bevacizumab Local Therapy: In Vitro Structural Features and Release Properties

Author(s)

Ferreira, Natalia Noronha ^[1] ; Caetano, Bruno Leonardo ^[1] ; Boni, Fernanda Isadora ^[1] ; Sousa, Flavia ^{[2, 3, 4, 5]} ; Magnani, Marina ^[6] ; Sarmento, Bruno ^{[2, 3, 4]} ; Ferreira Cury, Beatriz Stringhetti ^[1] ; Daflon Gremiao, Maria Palmira ^[1]

Affiliation

^[1] Univ Estadual Paulista UNESP, Fac Ciencias Farmaceut, Rodovia Araraquarae Jau, Km 1, BR-14801902 Araraquara, SP - Brazil

^[2] CESPU Inst Invest & Formacao Avancada Ciencias &, Rua Cent Gandra 1317, P-4585116 Gandra - Portugal

^[3] Univ Porto, I3S Inst Invest & Inovacao Saude, Rua Alfredo Allen 208, P-4200135 Porto - Portugal

^[4] Univ Porto, INEB Inst Engn Biomed, Rua Alfredo Allen 208, P-4200135 Porto - Portugal

^[5] Univ Porto, ICBAS, Rua Jorge Viterbo Ferreira 228, P-4050313 Porto - Portugal

^[6] Univ Estadual Paulista UNESP, Inst Quim, Rua Prof Francisco Degni 55, BR-14800060 Araraquara, SP - Brazil

• Document type: Journal article
• Source: Journal of Pharmaceutical Sciences; v. 108, n. 4, p. 1559-1568, APR 2019.
• Web of Science Citations: 1

Abstract

Alginate-based polyelectrolyte complexes (PECs) and hydrogel were engineered as platforms for local bevacizumab (BVZ) therapy. This study provides deep comprehension on the microstructures of such systems, and their correlation with drug-release patterns. PECs and hydrogel were characterized using Fourier transform infrared spectroscopy, small-angle X-ray scattering, scanning electron microscopy, atomic force microscopy, and porosimetry. Structural investigations indicated that PECs are formed by supramolecular interactions, resulting in physically cross-linked polymer networks, whereas the BVZ-loaded hydrogel has a more compact and rigid structure, promoting better entrapment of BVZ. PECs and hydrogel were able to control the BVZ release for 4 and 8 days, respectively. Their release profiles correlated best with the Higuchi and Korsmeyer-Peppas models, respectively, indicating drug diffusion as the limiting step for drug release. Furthermore, BVZ remained biologically active in vitro after its incorporation into the hydrogel system. Together, these studies confirm that PECs and hydrogel exhibit different porous structures and physicochemical properties, making them promising platforms that allow the modulation of BVZ release meeting different requirements. (c) 2019 American Pharmacists Association (R). Published by Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 14/50928-2 - INCT 2014: Pharmaceutical Nanotechnology: a transdisciplinary approach
• Grantee: Maria Vitória Lopes Badra Bentley
• Support Opportunities: Research Projects - Thematic Grants