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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Adaptive laboratory evolution of nanocellulose-producing bacterium

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Author(s):
Vasconcellos, Vanessa M. [1, 2] ; Farinas, Cristiane S. [1, 2] ; Ximenes, Eduardo [3] ; Slininger, Patricia [4] ; Ladisch, Michael [3]
Total Authors: 5
Affiliation:
[1] Embrapa Instrumentat, Sao Carlos, SP - Brazil
[2] Univ Fed Sao Carlos, Grad Program Chem Engn, Sao Carlos, SP - Brazil
[3] Purdue Univ, Lab Renewable Resources Engn, Weldon Sch Biomed Engn Agr & Biol Engn, W Lafayette, IN 47907 - USA
[4] USDA, Bioenergy Res Unit Anchor, Natl Ctr Agr Utilizat Res, Peoria, IL - USA
Total Affiliations: 4
Document type: Journal article
Source: Biotechnology and Bioengineering; v. 116, n. 8, p. 1923-1933, AUG 2019.
Web of Science Citations: 0
Abstract

Adaptive laboratory evolution through 12 rounds of culturing experiments of the nanocellulose-producing bacterium Komagataeibacter hansenii ATCC 23769 in a liquid fraction from hydrothermal pretreatment of corn stover resulted in a strain that resists inhibition by phenolics. The original strain generated nanocellulose from glucose in standard Hestrin and Schramm (HS) medium, but not from the glucose in pretreatment liquid. K. hansenii cultured in pretreatment liquid treated with activated charcoal to remove inhibitors also converted glucose to bacterial nanocellulose and used xylose as carbon source for growth. The properties of this cellulose were the same as nanocellulose generated from media specifically formulated for bacterial cellulose formation. However, attempts to directly utilize glucose proved unsuccessful due to the toxic character of the lignin-derived phenolics, and in particular, vanillan and ferulic acid. Adaptive laboratory evolution at increasing concentrations of pretreatment liquid from corn stover in HS medium resulted in a strain of K. hansenii that generated bacterial nanocellulose directly from pretreatment liquids of corn stover. The development of this adapted strain positions pretreatment liquid as a valuable resource since K. hansenii is able to convert and thereby concentrate a dilute form of glucose into an insoluble, readily recovered and value-added product-bacterial nanocellulose. (AU)

FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
Grantee:Roberto de Campos Giordano
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
FAPESP's process: 14/19000-3 - Simplification of the biomass to ethanol conversion process by integration with the production of enzymes in-house
Grantee:Cristiane Sanchez Farinas
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants