Effect of maternal periodontitis on GLUT4 and inflammatory pathway in adult offspring

Author(s):Show less -

de Lima Coutinho Mattera, Maria Sara ^[1] ; Chiba, Fernando Yamamoto ^[2] ; Lopes, Flavia Lombardi ^[3] ; Saori Tsosura, Thais Veronica ^[1] ; Peres, Maria Angelica ^[4] ; Balera Brito, Victor Gustavo ^[1] ; Penha de Oliveira, Sandra Helena ^[1] ; Pereira, Renato Felipe ^[1] ; Marani, Fernando ^[1] ; dos Santos, Rodrigo Martins ^[1] ; Belardi, Bianca Elvira ^[1] ; Lattari Tessarin, Gestter Willian ^{[5, 6]} ; Benites, Mariana Lopes ^[1] ; Ervolino, Edilson ^{[5, 6]} ; Heimann, Joel Claudio ^[4] ; Sumida, Doris Hissako ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Sch Dent, Dept Basic Sci, Multictr Postgrad Program Physiol Sci SBFis, Aracatuba - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Dent, Dept Child & Social Dent, Aracatuba - Brazil

^[3] Sao Paulo State Univ UNESP, Sch Vet Med, Aracatuba - Brazil

^[4] Univ Sao Paulo, Sch Med, Dept Internal Med, Lab Expt Hypertens, Sao Paulo - Brazil

^[5] Sao Paulo State Univ UNESP, Inst Biosci, Botucatu - Brazil

^[6] UNESP Univ Estadual Paulista, Dent Sch Aracatuba, Dept Basic Sci, Div Histol, Aracatuba - Brazil

• Document type: Journal article
• Source: Journal of Periodontology; v. 90, n. 8, p. 884-893, AUG 2019.
• Web of Science Citations: 0

Abstract

Background Maternal periodontal disease leads to low birth weight (LBW), insulin resistance (IR), increased TNF-alpha levels, and alterations in insulin signaling in adult offspring. TNF-alpha has been associated with the stimulation of IKK beta/NF-kappa B, resulting in the decreased expression of GLUT4. Another mechanism that may be involved in decreasing GLUT4 expression is DNA methylation. This study aimed to evaluate in the adult offspring of rats with periodontal disease: IR, inflammatory pathways, DNA methylation, and expression of GLUT4. Methods Female Wistar rats were distributed into control and experimental periodontal disease groups. Seven days after induction of periodontal disease, both groups were mated with healthy male rats. After weaning, male offspring were distributed into control offspring (CN-o) and periodontal disease offspring (PED-o) groups. Body weights were measured from 0-75 days of age. At day 75, the following were measured in the offspring: IR (HOMA-IR index); TNF-alpha and NF-kappa Bp65 content in the gastrocnemius muscle (GM) by western blotting; IKK alpha/beta, JNK, ERK 1/2, NF-kappa Bp65, and NF-kappa Bp50 phosphorylation status in the GM by western blotting; DNA methylation by restriction digest and real-time PCR(qAMP); and expression of GLUT4 mRNA in the GM by real-time PCR. Results LBW, IR, increases in TNF-alpha, IKK alpha/beta, ERK 1/2, NF-kappa Bp65, and NF-kappa Bp50 decreased expression of GLUT4 mRNA were observed in the PED-o rats. No differences were identified in JNK phosphorylation status and DNA methylation in the evaluated regions of the GLUT4-encoding gene Slc2a4. Conclusion Maternal periodontal disease causes LBW, IR, activation of inflammatory pathways, and decreased GLUT4 expression in the GM of adult offspring. (AU)

• FAPESP's process: 15/12018-7 - EVALUATION EPIGENETIC MECHANISM BY DNA METHYLATION AND EXPRESSION OF GLUT4 AND IRS-1 IN SKELETAL MUSCLE TISSUE OF ADULT RATS, OFFSPRING OF RATS WITH PERIODONTAL DISEASE
• Grantee: Maria Sara de Lima Coutinho Mattera
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/12405-6 - Effect of maternal periodontal disease in DNA methylation of GLUT4 and IRS-1 in skeletal muscle tissue of adult offspring
• Grantee: Bianca Elvira Belardi
• Support Opportunities: Scholarships in Brazil - Scientific Initiation