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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Layered Double Hydroxides Are Promising Nanomaterials for Tissue Bioengineering Application

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Fernandes, Celio Jr da Costa ; Pinto, Thais Silva [1] ; Kang, Ha Ram [1] ; Padilha, Pedro de Magalhaes [1] ; Koh, Ivan Hong Jun [2] ; Leopoldo Constantino, Vera Regina [3] ; Zambuzzi, Willian F. [1]
Total Authors: 7
[1] Fernandes, Jr., Celio Jr da Costa, Univ Estadual Paulista, UNESP, Lab Bioensaios & Dinam Celular, Inst Biociencias, Dept Quim & Bioquim, Campus Botucatu, BR-18618970 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, UNIFESP, Dept Cirurgia, Rua Botucatu 740, BR-04023900 Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Dept Quim Fundamental, Inst Quim, Av Prof Lineu Prestes 748, BR-05508000 Sao Paulo, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: ADVANCED BIOSYSTEMS; v. 3, n. 7 JUL 2019.
Web of Science Citations: 1

Layered double hydroxides (LDHs) have emerged as promising nanomaterials for human health and although it has achieved some progress on this matter, their application within bioengineering is not fully addressed. This prompted to subject fibroblasts to two compositions of LDHs (Mg2Al-Cl and Zn2Al-Cl), considering an acute response. First, LDH particles are addressed by scanning electron microscopy, and no significant effect of the cell culture medium on the shape of LDHs particles is reported although it seems to adsorb some soluble proteins as proposed by energy-dispersive X-ray analysis. These LDHs release magnesium, zinc, and aluminum, but there is no cytotoxic or biocompatibility effects. The data show interference to fibroblast adhesion by driving the reorganization of actin-based cytoskeleton, preliminarily to cell cycle progression. Additionally, these molecular findings are validated by performing a functional wound-healing assay, which is accompanied by a dynamic extracellular matrix remodeling in response to the LDHs. Altogether, the results show that LDHs nanomaterials modulate cell adhesion, proliferation, and migration, delineating new advances on the biomaterial field applied in the context of soft tissue bioengineering, which must be explored in health disorders, such as wound healing in burn injuries. (AU)

FAPESP's process: 14/22689-3 - Microvesicle/proteins-mediated paracrine signaling among bone and endothelial cells during bone development and regeneration
Grantee:Willian Fernando Zambuzzi
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 16/08888-9 - Microvesicle/proteins-mediated paracrine signaling among bone and endothelial cells during bone development and regeneration
Grantee:Célio Junior da Costa Fernandes
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)