Genetic variants of HIF1 alpha are associated with right ventricular fibrotic load in repaired tetralogy of Fallot patients: a cardiovascular magnetic resonance study

Author(s)

Hoang, Thanh T. ^[1] ; Manso, Paulo Henrique ^[2] ; Edman, Sharon ^[3] ; Mercer-Rosa, Laura ^{[4, 3]} ; Mitchell, Laura E. ^[1] ; Sewda, Anshuman ^[5] ; Swartz, Michael D. ^[6] ; Fogel, Mark A. ^{[4, 3]} ; Agopian, A. J. ^[1] ; Goldmuntz, Elizabeth ^{[4, 3]}

Affiliation

^[1] UTHlth Sch Publ Hlth, Dept Epidemiol Human Genet & Environm Sci, Houston, TX - USA

^[2] Univ Sao Paulo, Dept Pediat, Ribeiro Preto Med Sch, Ribeirao Preto - Brazil

^[3] Childrens Hosp Philadelphia, Div Cardiol, Abramson Res Ctr 702A, 3615 Civ Ctr Blvd, Philadelphia, PA 19104 - USA

^[4] Univ Penn, Dept Pediat, Perelman Sch Med, Philadelphia, PA 19104 - USA

^[5] Icahn Sch Med Mt Sinai, Dept Genet & Genom Sci, New York, NY 10029 - USA

^[6] UTHlth Sch Publ Hlth, Dept Biostat & Data Sci, Houston, TX - USA

• Document type: Journal article
• Source: JOURNAL OF CARDIOVASCULAR MAGNETIC RESONANCE; v. 21, n. 1 AUG 19 2019.
• Web of Science Citations: 0

Abstract

Background Studies suggest that right ventricular (RV) fibrosis is associated with RV remodeling and long-term outcomes in patients with tetralogy of Fallot (TOF). Pre-operative hypoxia may increase expression of hypoxia inducible factor-1-alpha (HIF1 alpha) and promote transforming growth factor beta 1 (TGF beta 1)-mediated fibrosis. We hypothesized that there would be associations between: (1) RV fibrosis and RV function, (2) HIF1 alpha variants and RV fibrosis, and (3) HIF1 alpha variants and RV function among post-surgical TOF cases. Methods We retrospectively measured post-surgical fibrotic load (indexed volume and fibrotic score) from 237 TOF cases who had existing cardiovascular magnetic resonance imaging using late gadolinium enhancement (LGE), and indicators of RV remodeling (i.e., ejection fraction {[}RVEF] and end-diastolic volume indexed {[}RVEDVI]). Genetic data were available in 125 cases. Analyses were conducted using multivariable linear mixed-effects regression with a random intercept and multivariable generalized Poisson regression with a random intercept. Results Indexed fibrotic volume and fibrotic score significantly decreased RVEF by 1.6% (p = 0.04) and 0.9% (p = 0.03), respectively. Indexed fibrotic volume and score were not associated with RVEDVI. After adjusting for multiple comparisons, 6 of the 48 HIF1 alpha polymorphisms (representing two unique signals) were associated with fibrotic score. None of the HIF1 alpha polymorphisms were associated with indexed fibrotic volume, RVEDVI, or RVEF. Conclusion The association of some HIF1 alpha polymorphisms and fibrotic score suggests that HIF1 alpha may modulate the fibrotic response in TOF. (AU)

• FAPESP's process: 14/12006-6 - Genetic determinants in right-ventricular fibrosis and remodeling after tetralogy of Fallot repair
• Grantee: Paulo Henrique Manso
• Support Opportunities: Scholarships abroad - Research