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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In vivo analysis of the presence of heme oxygenase-1, transcription factor Jun-D and CD90+/CD73+/CD105+/CD45-cells in the pulp of bleached teeth

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Author(s):
Benetti, F. [1, 2] ; Briso, A. L. F. [3] ; de Araujo Lopes, J. M. [2] ; Carminatti, M. [2] ; Conti, L. C. [2] ; Gallinari, M. O. [3] ; Ervolino, E. [4] ; Cintra, L. T. A. [2]
Total Authors: 8
Affiliation:
[1] Univ Fed Minas Gerais, Sch Dent, Dept Restorat Dent, Belo Horizonte, MG - Brazil
[2] Sao Paulo State Univ UNESP, Dept Endodont, Sch Dent, Aracatuba, SP - Brazil
[3] Sao Paulo State Univ UNESP, Dept Restorat Dent, Sch Dent, R Jose Bonifacio 1193, Aracatuba, SP - Brazil
[4] Sao Paulo State Univ UNESP, Dept Basic Sci, Sch Dent, Aracatuba, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: International Endodontic Journal; v. 52, n. 12 AUG 2019.
Web of Science Citations: 0
Abstract

Aim To investigate hydrogen peroxide (H2O2)-induced responsiveness in pulp cells using heme oxygenase-1 (HO-1) immunolabelling, Jun-D immunolabelling to study the effects of H2O2 on odontoblastic differentiation and CD90+/CD73+/CD105+/CD45- cell counting for in vivo identification of mesenchymal stem cells in the pulp. Methodology The maxillary molars of 50 rats were treated with a bleaching gel (35% H2O2, 1 x 30 min) or placebo gel (control groups). At 2, 3, 7, 15 and 30 days after the treatment (n = 10), inflammation in pulp tissue was analysed by haematoxylin-eosin staining, HO-1- and Jun-D-immunolabelled cells were counted in each third of the pulp chamber, and the number of CD90+/CD73+/CD105+/CD45- cells was quantified by immunofluorescence. The results were assessed using the Paired t-test or Wilcoxon signed-rank test (P < 0.05). Results Significant H2O2-induced inflammation was noted at 2 and 3 days (P < 0.05), with tertiary dentine formation occurring from 7 days. The bleached specimens had greater HO-1 immunolabelling in the middle and cervical thirds of the coronal pulp at 2 and 3 days, in all thirds at 7 days, and in the occlusal third at 15 days (P < 0.05), and significant nuclear Jun-D immunolabelling in the cervical third at 2 and 3 days and in the occlusal and middle thirds at 7 days (P < 0.05). Bleached and control groups had low numbers of CD90+/CD73+/CD105+/CD45- cells in the pulp at all periods (P > 0.05). Conclusions Pulp cells responded to oxidative stress by expressing HO-1 during the post-bleaching inflammation phase until the beginning of the repair phase. Jun-D expression occurred during the reduction of inflammation and the beginning of tertiary dentine production. The presence of oxidative stress did not influence the number of CD90+/CD73+/CD105+/CD45- cells identified in vivo in the dental pulp. (AU)

FAPESP's process: 15/10825-2 - Influence of Hydrogen Peroxide on cell differentiation and mineralization of the pulp tissue after bleaching procedure. Histological and immunohistochemical study.
Grantee:Francine Benetti
Support Opportunities: Scholarships in Brazil - Doctorate