Hydrogen, alcohols and volatile fatty acids from the co-digestion of coffee waste (coffee pulp, husk, and processing wastewater) by applying autochthonous microorganisms

Author(s)

Villa Montoya, Alejandra Carolina ^[1] ; da Silva Mazareli, Raissa Cristina ^[1] ; Delforno, Tiago Palladino ^[2] ; Centurion, Victor Bonin ^[2] ; Sakamoto, Isabel Kimiko ^[1] ; de Oliveira, Valeria Maia ^[2] ; Silva, Edson Luiz ^[3] ; Amancio Varesche, Maria Bernadete ^[1]

Affiliation

^[1] Univ Sao Paulo, Sao Carlos Sch Engn, Dept Hydraul & Sanitat, Lab Biol Proc, Campus 2, BR-13563120 Sao Carlos, SP - Brazil

^[2] Campinas Univ, Res Ctr Chem Biol & Agr CPQBA, Microbial Resources Div, BR-13081970 Campinas, SP - Brazil

^[3] Univ Fed Sao Carlos, Dept Chem Engn, Ctr Exact Sci & Technol, BR-13565905 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF HYDROGEN ENERGY; v. 44, n. 39, p. 21434-21450, AUG 13 2019.
• Web of Science Citations: 0

Abstract

The objective of this study was to screen the factors that affect H-2, organic acids and alcohols production from coffee waste pretreated in a hydrothermal reactor applying consortium of bacteria and fungi (indigenous from coffee waste) with hydrolytic and fermentative activity. The effects of pH (4.0-7.0), temperature (30-50 degrees C), agitation (0-180 rpm), headspace (50-70%), percentage of bioaugmentation (without microbial consortium to 20%), concentration of coffee pulp and husk (2-6 g/L), coffee processing wastewater (7-30 gCOD/L) and yeast extract (0-2 g/L) were evaluated using a Plackett-Burman design. The highest H-2 production potential (82 ml H-2) was obtained under the following conditions: 30 degrees C, 180 rpm, 50% headspace, without bioaugmentation, 2 g/L pulp and husk coffee, 30 gCOD/L coffee processing wastewater and 2 g/L yeast extract. The main soluble products were acetic acid (1956 mg/L), lactic acid (786 mg/L) and ethanol (816 mg/L). Lactobacillus sp., Clostridium sp., Saccharomyces sp. and Kazachstania sp. were the main autochthonous microorganisms identified. Through metagenome functional analysis, enzymes related to lignin, phenol, cellulose, lignocellulose, and pectin degradation were identified, as well as acidogenesis, and H-2 production. (C) 2019 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 16/20047-0 - Assessment of physicochemical and phylogenetic features in the hydrogen production and Homoacetogenesis from wastes of coffee processing
• Grantee: Alejandra Carolina Villa Montoya
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/06246-7 - Biorefinery concept applied to biological wastewater treatment plants: environmental pollution control coupled with material and energy recovery
• Grantee: Marcelo Zaiat
• Support type: Research Projects - Thematic Grants