Cholecalciferol Supplementation Did Not Change Interleukin-7 and B cell-Activating Factor Levels and CD95 Expression in B lymphocytes in Patients on Dialysis: A Randomized Pilot-Controlled Trial

Author(s)

Giffoni de Carvalho, Jose Tarcisio ^[1] ; Schneider, Marion ^[1] ; Cuppari, Lilian ^[1] ; Grabulosa, Caren Cristina ^[1] ; Cendoroglo, Miguel ^{[2, 1]} ; Dalboni, Maria Aparecida ^{[1, 3]}

Affiliation

^[1] Univ Fed Sao Paulo, UNIFESP, Div Nephrol, Sao Paulo, SP - Brazil

^[2] Hosp Israelita Albert Einstein, Sao Paulo, SP - Brazil

^[3] Univ Nove DeJulho UNINOVE, Postgrad Program Med, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF RENAL NUTRITION; v. 29, n. 5, p. 454-461, SEP 2019.
• Web of Science Citations: 1

Abstract

Background: End-stage renal disease results in B cell lymphopenia and low levels of vitamin D. Since the link between vitamin D deficiency and B lymphocytes dysfunction are not clear in patients with end-stage renal disease, we suggest that vitamin D adequacy and factors related to the homeostasis of these cells should be investigated. B lymphocytes homeostasis is a process mainly regulated signals of grown and death as interleukin (IL)-7, B cell-activating factor (BAFF)/BAFF-receptor and CD95 expression. Objective: As vitamin D serum levels were reduced in patients with end stage renal disease and it is associated with human B homeostasis, we evaluated the effect of cholecalciferol supplementation on dialysis. Design: Randomized, double blind clinical trial in dialysis patients with 250H Vitamin D deficiency for a period of 12 weeks. Main Outcome Measure: In a pilot study, we investigated the effect of cholecalciferol supplementation (100,000 UI once per week or placebo. In vitro, peripheral blood mononuclear cells isolated by Ficoll-Hypaque centrifugation from 12 healthy volunteers were incubated with healthy or uremic serum in the presence or absence of 25 (OH)DC with 5% CO. Results: There was an increase in the serum 25(OH)D level in the cholecalciferol group. No differences were found in BAFF and IL7 levels and CD95 and BAFF-R expression in B lymphocytes from patients on dialysis after cholecalciferol supplementation. Uremic serum induced an increase in the IL-7, BAFF, BAFF-R and CD95 expression compared with the control. However, we observed no effect of incubation of 25(OH)D3 and 1,25(OH)2D3 on the expression of IL-7, BAFF, BAFF-R and CD95 when incubated in the presence of normal or uremic serum. Conclusion: Our results suggest that vitamin D is not involved in mechanisms of regulation of differentiation and survival in B lymphocytes. In conclusion, further studies are needed to explore the effects of vitamin D on B lymphocytes to better evaluate the possible impact of vitamin D on humoral response in the CKD population. Published by Elsevier Inc. on behalf of the National Kidney Foundation, Inc. (AU)

• FAPESP's process: 12/21345-3 - Effects of cholecalciferol on modulation of expression of toll-like receptors (TLR-7 e TLR-9) and IL-6 e INF-gamma production in the b cells in uremia
• Grantee: Miguel Cendoroglo Neto
• Support type: Regular Research Grants
• FAPESP's process: 12/19314-2 - Effect of cholecalciferol on TLR-7 and TLR-9 expression and IL-6 and INF-y production by Lymphocytes B in uremia
• Grantee: José Tarcísio Giffoni de Carvalho Júnior
• Support type: Scholarships in Brazil - Doctorate