Inflammatory markers in the saliva of cerebral palsy individuals with gingivitis after periodontal treatment

Author(s)

Rosemeire Arai YOSHIDA ^[1] ; Renata GORJÃO ^[2] ; Marcia Pinto Alves MAYER ^[3] ; Paola Fernanda Leal CORAZZA ^[4] ; Renata Oliveira GUARE ^[5] ; Ana Cristina Fernandes Maria FERREIRA ^[6] ; Maria Teresa Botti Rodrigues SANTOS ^[7]

Affiliation

^[1] Universidade Cruzeiro do Sul. School of Dentistry - Brasil

^[2] Universidade Cruzeiro do Sul. Postgraduate Program Interdisciplinary in Health Sciences - Brasil

^[3] Universidade de São Paulo. Institute of Biomedical Sciences. Department of Microbiology - Brasil

^[4] Universidade Cruzeiro do Sul. School of Dentistry. Department of Periodontology - Brasil

^[5] Universidade Cruzeiro do Sul. School of Dentistry. Postgraduate Program in Dentistry - Brasil

^[6] Universidade Cruzeiro do Sul. School of Dentistry. Postgraduate Program in Dentistry - Brasil

^[7] Universidade Cruzeiro do Sul. Postgraduate Program in Dentistry - Brasil

• Document type: Journal article
• Source: Brazilian Oral Research; v. 33, 2019-07-01.

Abstract

Abstract The aim of this study was to evaluate the effect of periodontal treatment on the salivary cytokine levels and clinical parameters of individuals with cerebral palsy (CP) with gingivitis. A non-randomized, clinical trial was conducted in individuals diagnosed with spastic CP. Thirty-eight individuals were enrolled in the study and were categorized according to gingival index scores between 0-1 or 2-3, assigned to groups G2 or G1, respectively. Periodontal treatment comprised oral hygiene instructions, conventional mechanical treatment and 0.12% chlorhexidine applied as an adjunct. Clinical parameters and saliva samples were collected at baseline and at the 15-day follow-up visit. Bleeding on probing and periodontal screening and recording were determined. Non-stimulated saliva samples were obtained, and the salivary flow rate, the osmolality and the levels of cytokines IL-1β, IL-6, IL-8, IL-10, TNF-α and IL-12p70 were evaluated by a cytometric bead array. The Wilcoxon test, the Mann-Whitney test, Spearman correlation analysis, Poisson regression analysis and an adjusted analysis were performed (α = 0.05). The groups differed significantly in periodontal clinical parameters at baseline and at follow-up. Salivary flow rate and osmolality were similar in both groups at both timepoints. However, TNF-α and IL-1β levels were higher in G1 than in G2 at baseline. Mechanical treatment resulted in improved clinical parameters for both groups. Furthermore, mechanical treatment resulted in a significant reduction in salivary IL-1β and IL-8 levels for both groups after treatment. Periodontal treatment performed in individuals with CP and gingivitis reduces the levels of TNF-α, IL-1β, IL-6 and IL-8. (AU)

• FAPESP's process: 14/15662-1 - Effect of the photobiomodulation in the masseter muscle of children with cerebral palsy
• Grantee: Maria Teresa Botti Rodrigues dos Santos
• Support Opportunities: Regular Research Grants