| Full text | |
| Author(s): |
Inalda Angélica de Souza Ramos
[1]
;
Heitor Miraglia Herrera
[2]
;
Natália Serra Mendes
[3]
;
Simone de Jesus Fernandes
[4]
;
João Bosco Vilela Campos
[5]
;
João Vitor Almeida Alves
[6]
;
Gabriel Carvalho de Macedo
[7]
;
Rosangela Zacarias Machado
[8]
;
Marcos Rogério André
[9]
Total Authors: 9
|
| Affiliation: | [1] Universidade Estadual Paulista “Júlio de Mesquita Filho”. Faculdade de Ciências Agrárias e Veterinárias. Departamento de Patologia Veterinária - Brasil
[2] Universidade Católica Dom Bosco. Departamento de Parasitologia Veterinária - Brasil
[3] Universidade Estadual Paulista “Júlio de Mesquita Filho”. Faculdade de Ciências Agrárias e Veterinárias. Departamento de Patologia Veterinária - Brasil
[4] Universidade Estadual Paulista “Júlio de Mesquita Filho”. Faculdade de Ciências Agrárias e Veterinárias. Departamento de Patologia Veterinária - Brasil
[5] Universidade Católica Dom Bosco. Departamento de Parasitologia Veterinária - Brasil
[6] Universidade Católica Dom Bosco. Departamento de Parasitologia Veterinária - Brasil
[7] Universidade Católica Dom Bosco. Departamento de Parasitologia Veterinária - Brasil
[8] Universidade Estadual Paulista “Júlio de Mesquita Filho”. Faculdade de Ciências Agrárias e Veterinárias. Departamento de Patologia Veterinária - Brasil
[9] Universidade Estadual Paulista “Júlio de Mesquita Filho”. Faculdade de Ciências Agrárias e Veterinárias. Departamento de Patologia Veterinária - Brasil
Total Affiliations: 9
|
| Document type: | Journal article |
| Source: | Revista Brasileira de Parasitologia Veterinária; v. 28, n. 3, p. 451-457, 2019-08-01. |
| Abstract | |
Abstract The msp4 gene of A. marginale is unicodon, stable and mostly homogeneous, being considered as a useful marker for phylogeographic characterization of this bacterium. The objective of this work was to analyze the phylogeography of A. marginale based on the msp4 gene in beef cattle from the Brazilian Pantanal, compared to those found in other regions worldwide. The blood samples investigated were collected from 400 animals (200 cows and 200 calves) reared in five extensive breeding farms in this region. The results indicated that of the evaluated samples, 56.75% (227/400) were positive for A. marginale based on the msp1β gene by quantitatitve PCR (qPCR), while 8.37% (19/227) were positive for the msp4 gene in the conventional PCR. In the Network distance analysis, 14 sequences from the Brazilian Pantanal were grouped into a single group with those from Thailand, India, Spain, Colombia, Parana (Brazil), Mexico, Portugal, Argentina, China, Venezuela, Australia, Italy and Minas Gerais (Brazil). Among 68 sequences from Brazil and the world, 15 genotypes were present while genotype number one (#1) was the most distributed worldwide. Both Splitstree and network analyses showed that the A. marginale msp4 sequences detected in beef cattle from the Brazilian Pantanal showed low polymorphism, with the formation of one genogroup phylogenetically related to those found in ruminants from South and Central America, Europe, and Asia. (AU) | |
| FAPESP's process: | 15/14896-1 - Molecular detection and characterization of agents belonging to Anaplasmataceae e Bartonellaceae family in wild animals and ectoparasites in Brazil |
| Grantee: | Marcos Rogério André |
| Support Opportunities: | Regular Research Grants |