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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The E3 ligase MuRF2 plays a key role in the functional capacity of skeletal muscle fibroblasts

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Author(s):
J.G. Silvestre [1] ; I.L. Baptista [2] ; W.J. Silva [3] ; A. Cruz [4] ; M.T. Silva [5] ; E.H. Miyabara [6] ; S. Labeit [7] ; A.S. Moriscot [8]
Total Authors: 8
Affiliation:
[1] Instituto de Ciências Biomédicas, Universidade de São Paulo. Departamento de Anatomia - Brasil
[2] Faculdade de Ciências Aplicadas, UNICAMP - Brasil
[3] Instituto de Ciências Biomédicas, Universidade de São Paulo. Departamento de Anatomia - Brasil
[4] Instituto de Ciências Biomédicas, Universidade de São Paulo. Departamento de Anatomia - Brasil
[5] Instituto de Ciências Biomédicas, Universidade de São Paulo. Departamento de Anatomia - Brasil
[6] Instituto de Ciências Biomédicas, Universidade de São Paulo. Departamento de Anatomia - Brasil
[7] Faculty for Clinical Medicine Mannheim, University of Heidelberg. Institute for Integrative Pathophysiology, Mannheim Medical University - Alemanha
[8] Instituto de Ciências Biomédicas, Universidade de São Paulo. Departamento de Anatomia - Brasil
Total Affiliations: 8
Document type: Journal article
Source: Brazilian Journal of Medical and Biological Research; v. 52, n. 9 2019-08-29.
Abstract

Fibroblasts are a highly heterogeneous population of cells, being found in a large number of different tissues. These cells produce the extracellular matrix, which is essential to preserve structural integrity of connective tissues. Fibroblasts are frequently engaged in migration and remodeling, exerting traction forces in the extracellular matrix, which is crucial for matrix deposition and wound healing. In addition, previous studies performed on primary myoblasts suggest that the E3 ligase MuRF2 might function as a cytoskeleton adaptor. Here, we hypothesized that MuRF2 also plays a functional role in skeletal muscle fibroblasts. We found that skeletal muscle fibroblasts express MuRF2 and its siRNA knock-down promoted decreased fibroblast migration, cell border accumulation of polymerized actin, and down-regulation of the phospho-Akt expression. Our results indicated that MuRF2 was necessary to maintain the actin cytoskeleton functionality in skeletal muscle fibroblasts via Akt activity and exerted an important role in extracellular matrix remodeling in the skeletal muscle tissue. (AU)

FAPESP's process: 16/12941-2 - Identification and characterization of leucine responsive mRNAs in skeletal muscle of rats subjected to immobilization with large-scale sequencing
Grantee:João Guilherme de Oliveira Silvestre
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 15/04090-0 - Identification and caracterization of mechanisms involved in skeletal muscle mass control and regeneration
Grantee:Anselmo Sigari Moriscot
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 12/13315-7 - Role of MuRF1 and MuRF2 in skeletal muscle myogenic cell differentiation
Grantee:João Guilherme de Oliveira Silvestre
Support Opportunities: Scholarships in Brazil - Doctorate