Scale-up of plants production of native palm trees economically important
Scale-up of plants production of native palm trees economically important
Genomics and conservation genetics of populations of juçara (Euterpe edulis Martiu...
Full text | |
Author(s): |
Marília Souza Lucas
[1]
;
Carolina da Silva Carvalho
;
Giovane Böerner Hypolito
[3]
;
Marina Corrêa Côrtes
[4]
Total Authors: 4
|
Affiliation: | [1] Universidade do Estado de São Paulo. Instituto de Biociências. Departamento de Ecologia - Brasil
[3] Universidade do Estado de São Paulo. Instituto de Biociências. Departamento de Ecologia - Brasil
[4] Universidade do Estado de São Paulo. Instituto de Biociências. Departamento de Ecologia - Brasil
Total Affiliations: 4
|
Document type: | Journal article |
Source: | Hoehnea; v. 46, n. 2 2019-07-22. |
Abstract | |
ABSTRACT The application of molecular techniques to tackle ecological and evolutionary questions requires genomic DNA in good quality and quantity. The quality of the isolated DNA, however, can be influenced by the tissue type and the way the sample was conserved and manipulated. Therefore, customizing protocols to improve the DNA isolation and locus amplification is crucial. We optimized a cheap and manual protocol of DNA extraction and microsatellites amplification using five different tissues of a palm species of the brazilian Atlantic Forest. We successfully extracted DNA from all five tissue types. Leaf, stem, and endocarp of non-dispersed seeds presented the highest rates of successful DNA extraction and microsatellite amplification; whereas root, endocarp of dispersed seeds, and embryo showed the lowest quality and quantity. Based on these results, we discussed the implications of using different tissues for studies about seed dispersal, pollination, and population genetics. (AU) | |
FAPESP's process: | 16/18235-2 - Pollen dispersal and reproductive system of the palm Euterpe edulis in Atlantic Forest fragments |
Grantee: | Giovane Böerner Hypolito |
Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
FAPESP's process: | 14/01029-5 - Ecological and genetics effects of seed size variation in a defaunated landscape |
Grantee: | Carolina da Silva Carvalho |
Support Opportunities: | Scholarships in Brazil - Doctorate |