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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Prostaglandin E2 Induces Expression of Mineralization Genes by Undifferentiated Dental Pulp Cells

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Author(s):
Francine Lorencetti-Silva [1] ; Priscilla Aparecida Tartari Pereira [2] ; Alyne Fávero Galvão Meirelles [3] ; Lúcia Helena Faccioli [4] ; Francisco Wanderley Garcia Paula-Silva
Total Authors: 5
Affiliation:
[1] USP - Universidade de São Paulo. School of Dentistry of Ribeirão Preto. Department of Pediatric Clinics - Brasil
[2] Universidade de São Paulo. Toxicológicas e Bromatológicas da Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas - Brasil
[3] Universidade de São Paulo. Toxicológicas e Bromatológicas da Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas - Brasil
[4] Universidade de São Paulo. Toxicológicas e Bromatológicas da Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas - Brasil
Total Affiliations: 5
Document type: Journal article
Source: Brazilian Dental Journal; v. 30, n. 3, p. 201-207, 2019-06-03.
Abstract

Abstract Prostaglandin E2 (PGE2) is a lipid mediator usually released during inflammation. This study aimed to investigate the potential of soluble or microsphere-loaded PGE2 on inducing differentiation of dental pulp stem cells. PGE2-loaded microspheres (MS) were prepared using an oil-in-water emulsion solvent extraction-evaporation process and were characterized. Mouse dental pulp stem cells (OD-21) were stimulated with soluble or PGE2-loaded MS (0.01 and 0.1 µM). Cell viability was determined by MTT colorimetric assay. Ibsp, Bmp2 and Runx2 expression was measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR) after 3, 6, and 24 h. The results showed that the soluble PGE2 reduced dental pulp stem cells viability after 24 h of stimulation whereas PGE2-loaded MS did not. Soluble PGE2 up-regulated Ibsp and Bmp2 at 3 h, differently from PGE2-loaded MS. On the other hand, PGE2-MS induced Bmp2 and Runx2 at 6 h and Ibsp at 24 h. In conclusion, our in vitro results show that PGE2, soluble or loaded in MS are not cytotoxic and modulateIbsp,Bmp2, andRunx2gene expression in cultured OD-21 cells. (AU)

FAPESP's process: 10/17611-4 - Mechanisms involved in the regulation of 5-lipoxygenase pathway in experimentally-induced apical periodontitis
Grantee:Francisco Wanderley Garcia de Paula e Silva
Support Opportunities: Research Grants - Young Investigators Grants