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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

New Peroxisome Proliferator-Activated Receptor Agonist (GQ-11) Improves Wound Healing in Diabetic Mice

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Author(s):
Silva, Jacqueline C. [1] ; Pitta, Marina G. R. [2] ; Pitta, Ivan R. [2] ; Koh, Timothy J. [3] ; Abdalla, Dulcineia S. P. [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin & Toxicol Anal, Ave Prof Lineu Prestes 580, BR-05508000 Sao Paulo, SP - Brazil
[2] Univ Fed Pernambuco, Ctr Biol Sci, Core Therapeut Innovat, Recife, PE - Brazil
[3] Univ Illinois, Coll Appl Hlth Sci, Dept Kinesiol & Nutr, 1919 West Taylor St, 529 AHSB, Chicago, IL 60612 - USA
Total Affiliations: 3
Document type: Journal article
Source: ADVANCES IN WOUND CARE; v. 8, n. 9, p. 417-428, SEP 1 2019.
Web of Science Citations: 0
Abstract

Objective: Chronic wounds associated with diabetes are an important public health problem demanding new treatments to improve wound healing and decrease amputations. Monocytes/macrophages play a key role in sustained inflammation associated with impaired healing and local administration of peroxisome proliferator-activated receptor (PPAR)gamma agonists may modulate macrophage, improving healing. In this study, we investigated the effects of GQ-11, a partial/dual PPAR alpha/gamma agonist, on macrophage function and wound healing in diabetes. Approach: Wounds were surgically induced at the dorsum of C57BL/6J and BKS.Cg-Dock7(m) +/+ Lepr(db)/J (db/db) mice and treated with hydrogel (vehicle), pioglitazone or GQ-11, for 7 or 10 days, respectively. After treatment, wounds were analyzed histologically and by quantitative PCR (qPCR). In addition, bone marrow-derived macrophages (BMDM) were cultured from C57BL/6J mice and treated with vehicle, pioglitazone, or GQ-11, after challenge with lipopolysaccharide or interleukin-4 to be analyzed by qPCR and flow cytometry. Results: GQ-11 treatment upregulated anti-inflammatory/pro-healing factors and downregulated pro-inflammatory factors both in wounds of db/db mice and in BMDM. Innovation: Wounds of db/db mice treated with GQ-11 exhibited faster wound closure and re-epithelization, increased collagen deposition, and less Mac-3 staining compared with vehicle, providing a new approach to treatment of diabetic wound healing to prevent complications. Conclusion: GQ-11 improves wound healing in db/db mice, regulating the expression of pro- and anti-inflammatory cytokines and wound growth factors, leading to increased re-epithelization and collagen deposition. (AU)

FAPESP's process: 12/51316-5 - Study of the activity of biodrugs, PPARs agonists and natural products with therapeutic potential in atherosclerosis
Grantee:Dulcineia Saes Parra Abdalla
Support type: Research Projects - Thematic Grants
FAPESP's process: 16/00233-3 - "Action of a novel thiazolidinedione (GQ-11) in tissue repair process on experimental models of insulin resistance and vascular surgery.
Grantee:Jacqueline Cavalcante Silva
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 16/16850-1 - Modulation of tissue repair by a new thiazolidine compound (GQ-11) on experimental models of insulin resistance and visceral ischemia
Grantee:Jacqueline Cavalcante Silva
Support type: Scholarships abroad - Research Internship - Doctorate