| Full text | |
| Author(s): |
Silva, Jacqueline C.
[1]
;
Pitta, Marina G. R.
[2]
;
Pitta, Ivan R.
[2]
;
Koh, Timothy J.
[3]
;
Abdalla, Dulcineia S. P.
[1]
Total Authors: 5
|
| Affiliation: | [1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin & Toxicol Anal, Ave Prof Lineu Prestes 580, BR-05508000 Sao Paulo, SP - Brazil
[2] Univ Fed Pernambuco, Ctr Biol Sci, Core Therapeut Innovat, Recife, PE - Brazil
[3] Univ Illinois, Coll Appl Hlth Sci, Dept Kinesiol & Nutr, 1919 West Taylor St, 529 AHSB, Chicago, IL 60612 - USA
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | ADVANCES IN WOUND CARE; v. 8, n. 9, p. 417-428, SEP 1 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
Objective: Chronic wounds associated with diabetes are an important public health problem demanding new treatments to improve wound healing and decrease amputations. Monocytes/macrophages play a key role in sustained inflammation associated with impaired healing and local administration of peroxisome proliferator-activated receptor (PPAR)gamma agonists may modulate macrophage, improving healing. In this study, we investigated the effects of GQ-11, a partial/dual PPAR alpha/gamma agonist, on macrophage function and wound healing in diabetes. Approach: Wounds were surgically induced at the dorsum of C57BL/6J and BKS.Cg-Dock7(m) +/+ Lepr(db)/J (db/db) mice and treated with hydrogel (vehicle), pioglitazone or GQ-11, for 7 or 10 days, respectively. After treatment, wounds were analyzed histologically and by quantitative PCR (qPCR). In addition, bone marrow-derived macrophages (BMDM) were cultured from C57BL/6J mice and treated with vehicle, pioglitazone, or GQ-11, after challenge with lipopolysaccharide or interleukin-4 to be analyzed by qPCR and flow cytometry. Results: GQ-11 treatment upregulated anti-inflammatory/pro-healing factors and downregulated pro-inflammatory factors both in wounds of db/db mice and in BMDM. Innovation: Wounds of db/db mice treated with GQ-11 exhibited faster wound closure and re-epithelization, increased collagen deposition, and less Mac-3 staining compared with vehicle, providing a new approach to treatment of diabetic wound healing to prevent complications. Conclusion: GQ-11 improves wound healing in db/db mice, regulating the expression of pro- and anti-inflammatory cytokines and wound growth factors, leading to increased re-epithelization and collagen deposition. (AU) | |
| FAPESP's process: | 12/51316-5 - Investigacao da atividade de biofarmacos,agonistas de ppars e produtos naturais com potencial terapeutico na aterosclerose. |
| Grantee: | Dulcineia Saes Parra Abdalla |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 16/00233-3 - "Action of a novel thiazolidinedione (GQ-11) in tissue repair process on experimental models of insulin resistance and vascular surgery. |
| Grantee: | Jacqueline Cavalcante Silva |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 16/16850-1 - Modulation of tissue repair by a new thiazolidine compound (GQ-11) on experimental models of insulin resistance and visceral ischemia. |
| Grantee: | Jacqueline Cavalcante Silva |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |