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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Bioglass/PLGA associated to photobiomodulation: effects on the healing process in an experimental model of calvarial bone defect

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Paiva Magri, Angela Maria [1, 2] ; Fernandes, Kelly Rossetti [1] ; Kido, Hueliton Wilian [1] ; Fernandes, Gabriela Sodano [1] ; Fermino, Stephanie de Souza [1] ; Gabbai-Armelin, Paulo Roberto [1] ; Correa Braga, Franscisco Jose [3] ; Goes, Antia Pereirade [1] ; dos Santos Prado, Jose Lucas [1] ; Granito, Renata Neves [1] ; Muniz Renno, Ana Claudia [1]
Total Authors: 11
[1] Fed Univ Sao Paulo UNIFESP, Rua Silva Jardim 136, BR-11015020 Santos, SP - Brazil
[2] Guaxupe Educ Fdn UNIFEG, Univ Ctr, Ave Dona Floriana, BR-37800000 Guaxupe, MG - Brazil
[3] Consulmat LTDA, Rua Juan Lopes 159, BR-13567020 Sao Carlos, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Web of Science Citations: 0

Bioactive glasses (BG) are known for their ability to bond to bone tissue. However, in critical situations, even the osteogenic properties of BG may be not enough to induce bone consolidation. Thus, the enrichment of BG with polymers such as Poly (D, L-lactic-co-glycolic) acid (PLGA) and associated to photobiomodulation (PBM) may be a promising strategy to promote bone tissue healing. The aim of the present study was to investigate the in vivo performance of PLGA supplemented BG, associated to PBM therapy, using an experimental model of cranial bone defect in rats. Rats were distributed in 4 different groups (Bioglass, Bioglass/PBM, Bioglas/PLGA and BG/PLGA/PBM). After the surgical procedure to induce cranial bone defects, the pre-set samples were implanted and PBM treatment (low-level laser therapy) started (808 nm, 100 mW, 30 J/cm(2)). After 2 and 6 weeks, animals were euthanized, and the samples were retrieved for the histopathological, histomorphometric, picrosirius red staining and immunohistochemistry analysis. At 2 weeks post-surgery, it was observed granulation tissue and areas of newly formed bone in all experimental groups. At 6 weeks post-surgery, BG/PLGA (with or without PBM) more mature tissue around the biomaterial particles. Furthermore, there was a higher deposition of collagen for BG/PLGA in comparison with BG/PLGA/PBM, at second time-point. Histomorphometric analysis demonstrated higher values of BM.V/TV for BG compared to BG/PLGA (2 weeks post-surgery) and N.Ob/T.Ar for BG/PLGA compared to BG and BG/PBM (6 weeks post-surgery). This current study concluded that the use of BG/PLGA composites, associated or not to PBM, is a promising strategy for bone tissue engineering. (AU)

FAPESP's process: 14/20546-0 - Lasertherapy and Biosilicate/magnesium/collagen composites, used in association or isolated: in vitro and in vivo biological evaluation in bone tissue
Grantee:Ana Claudia Muniz Renno
Support type: Regular Research Grants