Effects of photobiomodulation in association with composites of spongine extracted from marine sponges and biosilicate during the bone repair

• Grant number: 18/04792-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2018
• End date: November 30, 2019
• Field of knowledge: Interdisciplinary Subjects
• Principal Investigator: Ana Claudia Muniz Renno
• Grantee: Giovanna Caroline Aparecida do Vale
• Host Institution: Instituto de Saúde e Sociedade (ISS). Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil

Abstract

Fractures that affect the bone system have been increasing significantly, related to a high socioeconomic cost and high rates of morbidity and mortality. In this context, the bone tissue engineering has been developing new biomaterials and therapeutic resources improve the repair of bone fractures. Among the treatment methods, the use of photobiomodulation associated with the use of biomaterials stands out. In this context, the marine ecosystem constitutes a rich environment for the supply of raw material to develop biotechnological products to be used in bone repair. One of the target marine organisms is marine sponges, which have a component called spongine in their skeleton, with enormous potential to optimize the bone repair. Still, Biosilicate is a glass ceramic that presents effective and promising results during the process of bone repair. In this way, the objective of this project is to evaluate in vivo tests the effects of photobiomodulation associated or not with Biosilicate/Spongine composites in the bone repair of defects induced in calvarial bone defect model. For this, 32 Wistar rats submitted to the surgical procedure to perform the calvarial bone defect of rats will be used. The defect will be filled with the different biomaterials, associated or not with photobiomodulation (808 nm, 30 mW, 1.07 W/cm2, 30 J/cm2 and 28 seconds). Still, the animals will be euthanized after 15 and 45 days of surgery. Histopathology, morphometry and immunohistochemistry analyzes will be performed. This project will contribute to the development of new technologies to be used in bone repair from national and natural biomaterials associated with photobiomodulation.