| Full text | |
| Author(s): Show less - |
dos Santos, Nilton B.
[1]
;
Franco, Roseane D.
[1]
;
Camarini, Rosana
[1]
;
Munhoz, Carolina D.
[1]
;
Eichler, Rosangela A. S.
[1]
;
Gewehr, Mayara C. F.
[1]
;
Reckziegel, Patricia
[1, 2]
;
Llanos, Ricardo P.
[1]
;
Dale, Camila S.
[3]
;
da Silva, Victoria R. O.
[3]
;
Borges, Vanessa F.
[4]
;
Lima, Braulio H. F.
[4]
;
Cunha, Fernando Q.
[4]
;
Visniauskas, Bruna
[5]
;
Chagas, Jair R.
[5]
;
Tufik, Sergio
[5]
;
Peres, Fernanda F.
[2]
;
Abilio, Vanessa C.
[2]
;
Florio, Jorge C.
[6]
;
Iwai, Leo K.
[7]
;
Rioli, Vanessa
[7]
;
Presoto, Benedito C.
[8]
;
Guimaraes, Alessander O.
[9]
;
Pesquero, Joao B.
[9]
;
Bader, Michael
[10, 11, 12, 13, 14]
;
Castro, Leandro M.
[15]
;
Ferro, Emer S.
[1]
Total Authors: 27
|
| Affiliation: Show less - | [1] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, BR-05508000 Sao Paulo, SP - Brazil
[2] Fed Univ Sao Paulo UNIFESP, Dept Pharmacol, BR-04023062 Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, BR-05508000 Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Pharmacol, BR-14049900 Ribeirao Preto, SP - Brazil
[5] Fed Univ Sao Paulo UNIFESP, Dept Psychobiol, BR-04023062 Sao Paulo - Brazil
[6] Univ Sao Paulo, Vet Med Sch, Dept Pathol, BR-05508000 Sao Paulo, SP - Brazil
[7] Butantan Inst, Ctr Toxins Immune Response & Cell Signaling CETIC, Special Lab Appl Toxinol LETA, BR-05503900 Sao Paulo - Brazil
[8] Butantan Inst, Pharmacol Lab, BR-05503900 Sao Paulo - Brazil
[9] Fed Univ Sao Paulo UNIFESP, Dept Biophys, BR-04023062 Sao Paulo, SP - Brazil
[10] Max Delbruck Ctr Mol Med, D-13125 Berlin - Germany
[11] Charite Univ Med Berlin, D-13353 Berlin - Germany
[12] BIH, D-10117 Berlin - Germany
[13] DZHK German Ctr Cardiovasc Res, Partner Site Berlin, D-10785 Berlin - Germany
[14] Univ Lubeck, Inst Biol, D-23538 Lubeck - Germany
[15] Sao Paulo State Univ UNESP, Biosci Inst, BR-11330900 Sao Vicente, SP - Brazil
Total Affiliations: 15
|
| Document type: | Journal article |
| Source: | BIOMOLECULES; v. 9, n. 8 AUG 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
Thimet oligopeptidase (THOP1) is thought to be involved in neuropeptide metabolism, antigen presentation, neurodegeneration, and cancer. Herein, the generation of THOP1 C57BL/6 knockout mice (THOP1(-/-)) is described showing that they are viable, have estrus cycle, fertility, and a number of puppies per litter similar to C57BL/6 wild type mice (WT). In specific brain regions, THOP1(-/-) exhibit altered mRNA expression of proteasome beta5, serotonin 5HT2a receptor and dopamine D2 receptor, but not of neurolysin (NLN). Peptidomic analysis identifies differences in intracellular peptide ratios between THOP1(-/-) and WT mice, which may affect normal cellular functioning. In an experimental model of multiple sclerosis THOP1(-/-) mice present worse clinical behavior scores compared to WT mice, corroborating its possible involvement in neurodegenerative diseases. THOP1(-/-) mice also exhibit better survival and improved behavior in a sepsis model, but also a greater peripheral pain sensitivity measured in the hot plate test after bradykinin administration in the paw. THOP1(-/-) mice show depressive-like behavior, as well as attention and memory retention deficits. Altogether, these results reveal a role of THOP1 on specific behaviors, immune-stimulated neurodegeneration, and infection-induced inflammation. (AU) | |
| FAPESP's process: | 16/04000-3 - Pharmacology of oligopeptidases and intracellular peptides |
| Grantee: | Emer Suavinho Ferro |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 15/07273-8 - Molecular mechanism of intracellular peptides on gene expression and signaling in skeletal muscle model |
| Grantee: | Ricardo Pariona Llanos |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 04/04933-2 - Molecular cell biology of oligopeptidases |
| Grantee: | Emer Suavinho Ferro |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 15/20657-0 - Evaluation of hemopressin (HP) pharmacological use on obesity treatment |
| Grantee: | Patrícia Reckziegel |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |