PLGA-PEG nanoparticles containing gallium phthalocyanine: Preparation, optimization and analysis of its photodynamic efficiency on red blood cell and Hepa-1C1C7

Author(s)

Lorenzoni, Drielly ^[1] ; Zanoni Souto, Carlos Augusto ^[1] ; Araujo, Maiara Barboza ^[1] ; Berger, Carol de Souza ^[1] ; Duarte da Silva, Luciene Cristina ^[1] ; Baratti, Mariana Ozello ^[2] ; Ribeiro, Joselito Nardy ^[3] ; Endringer, Denise Coutinho ^[4] ; Cunegundes Guimaraes, Marco Cesar ^[3] ; da Silva, Andre Romero ^[1]

Affiliation

^[1] Fed Inst Espirito Santo, Campus Aracruz, Moroba Ave, 248, BR-29192733 Aracruz, ES - Brazil

^[2] Univ Estadual Campinas, Dept Cellular Biol, BR-13083863 Campinas, SP - Brazil

^[3] Univ Fed Espirito Santo, Campus Maruipe, BR-29047105 Vitoria, ES - Brazil

^[4] Univ Vila Velha, Campus Vista, BR-29102920 Vila Velha, ES - Brazil

• Document type: Journal article
• Source: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 198, SEP 2019.
• Web of Science Citations: 0

Abstract

Poly(lactide-co-glycolide) (PLGA) has been used for the encapsulation of phthalocyanine motived by its biocompatibility and biodegradability. Many studies have already been done to evaluate the influence of parameters used in the PLGA nanoparticle synthesis but without the evaluation of the combinatory interaction between these parameters on the nanoparticulate properties. Ga(III)-phthalocyanine (GaPc) was encapsulated into the PEGlated PLGA-nanoparticles and the individual and combinatory effects of the emulsification time, the method used for the nanoparticle synthesis and the temperature of the aqueous phase was evaluated on the size, entrapment efficiency, efficacy of nanoparticle recovery, residual PVA and zeta potential value using a 2(3) factorial design (FD). Mathematical models were adjustable to the data and evolutionary operations were performed to optimize the nanoparticle size. The ability of the optimized nanoparticle to decrease the viability of the Hepa-1C1C7 cell and the blood red cell was also evaluated. The FD disclosed the emulsification-diffusion method decreased the residual PVA and the size of PLGA-PEG nanoparticle, but also decreased the entrapment efficiency of GaPc, the zeta potential absolute value and the recovery efficacy of nanoparticles. The combinatory effect between the method used in the nanoparticle preparation and the temperature of aqueous phase influenced four of the five evaluated properties. The viability of Hepa-1C1C7 cells was reduced until 13 x when the cells were irradiated in the presence of encapsulated GaPc while it was decreased until 4.7 x when the experiment was carried out with the free GaPc. The encapsulated GaPc was also more efficient to cause the haemolysis of the RBC than it was the free GaPc. The optimization of the nanoparticles synthesis increased the efficiency of the GaPc to oxidize the evaluated cells. (AU)

• FAPESP's process: 14/50938-8 - INCT 2014: in Photonics Applied to Cell Biology
• Grantee: Hernandes Faustino de Carvalho
• Support Opportunities: Research Projects - Thematic Grants