Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

STAT1-NF kappa B crosstalk triggered by interferon gamma regulates noradrenaline-induced pineal hormonal production

Full text
Author(s):
Barbosa Lima, Leila Eliza [1] ; Muxel, Sandra Marcia [1] ; Kinker, Gabriela S. [1] ; Carvalho-Sousa, Claudia Emanuele [1] ; Cruz-Machado, Sanseray da Silveira [1] ; Markus, Regina P. [1] ; Carlos Magno Fernandes, Pedro Augusto [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Inst Biosci, Dept Physiol, Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Journal of Pineal Research; v. 67, n. 3 OCT 2019.
Web of Science Citations: 1
Abstract

Melatonin production by pineal glands is modulated by several immune signals. The nuclear translocation of nuclear factor kappa-B (NF kappa B) homodimers, lacking transactivation domains, once induced by lipopolysaccharide (LPS) or tumor necrosis factor (TNF), inhibits the expression of Aanat gene and the synthesis of noradrenaline (NA)-induced melatonin. Interferon gamma (IFN-gamma), on the other hand, increases melatonin synthesis. Furthermore, this cytokine activates the signal transducer as well as the activator of transcription 1 (STAT1) pathway, which was never evaluated as a melatonin synthesis modulator before. Reports demonstrated that IFN-gamma might also activate NF kappa B. The present study evaluated the role of STAT1-NF kappa B crosstalk triggered by IFN-gamma regarding the regulation of NA-induced pineal glands' hormonal production. Moreover, IFN-gamma treatment increased NA-induced Aanat transcription, in addition to the synthesis of N-acetylserotonin (NAS) and melatonin. These effects were associated with STAT1 nuclear translocation, confirmed by the co-immunoprecipitation of STAT1 and Aanat promoter. Pharmacological STAT1 enhancement augmented NA-induced Aanat transcription as well as NAS and melatonin production. Additionally, IFN-gamma induced the nuclear translocation of RelA-NF kappa B subunits. The blockade of this pathway prevented IFN-gamma effects on the pineal function. The present data show that STAT1 and NF kappa B crosstalk controls melatonin production through a synergistic mechanism, disclosing a new integrative mechanism regarding pineal hormonal activity control. (AU)

FAPESP's process: 12/09622-1 - Characterization of the molecular effects of interferon-gamma on the production of melatonin by rat pineal glands.
Grantee:Leila Eliza Barbosa Lima
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 10/52687-1 - Characterization of the role of adrenal/pineal interaction in the immune-pineal axis context
Grantee:Pedro Augusto Carlos Magno Fernandes
Support Opportunities: Regular Research Grants
FAPESP's process: 13/13691-1 - Immune-pineal axis: time-niology integrated to surveillance and defense
Grantee:Regina Pekelmann Markus
Support Opportunities: Research Projects - Thematic Grants