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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

M4-muscarinic acetylcholine receptor into the pedunculopontine tegmental nucleus mediates respiratory modulation of conscious rats

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Lima, Janayna D. [1] ; Sobrinho, Cleyton R. [1] ; Santos, Leonardo K. [1] ; Takakura, Ana C. [2] ; Moreira, Thiago S. [1]
Total Authors: 5
[1] Univ Sao Paulo, Dept Physiol & Biophys, BR-05508 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Dept Pharmacol, BR-05508 Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Respiratory Physiology & Neurobiology; v. 269, NOV 2019.
Web of Science Citations: 0

The pedunculopontine tegmental nucleus (PPTg) has been shown to have important functions relevant to the regulation of behavioral states and various motor control systems, including breathing control. The PPTg is considered an important nucleus in the mesopontine region with considerably cholinergic input to the ventral respiratory column. In addition, recent studies indicate that cholinergic innervation of the ventral respiratory column may play an important role in modulation of breathing. Here, we investigated the cholinergic stimulation of the PPTg and the changes in breathing output in conscious rats. Male Wistar rats (280-350 g, N = 5-12/group) with unilateral stainless steel cannula implanted into the PPTg were used. Respiratory parameters (tidal volume (V-T), respiratory frequency (f(R)) and ventilation (V-E)) were analyzed by whole body plethysmography. In unrestrained awake rats, unilateral injection of the cholinergic muscarinic agonist carbachol (10 mM-100 nL) in the PPTg decreased f(R), and increase V-T, without changing V-E. The changes in f(R) and V-T elicited by carbachol into the PPTg are abolished by previous blockade of the M4 muscarinic cholinergic receptors tropicamide into the PPTg. No significant changes in f(R) and V-T elicited by carbachol were observed after blockade of the M1 and/or M3 muscarinic cholinergic receptors pirenzepine or 4-DAMP into the PPTg. Our data suggest that the changes in f(R) and V-T produced by muscarinic cholinergic stimulation of PPTg is presumably mediated through a Gi-coupled M4 muscarinic receptors. (AU)

FAPESP's process: 16/23281-3 - Encephalic regions responsible for neuroplasticity observed in respiratory response induced by hypercapnia in a modelo of Parkinson's Disease
Grantee:Ana Carolina Thomaz Takakura
Support type: Regular Research Grants
FAPESP's process: 15/23376-1 - Retrotrapezoid nucleus, respiratory chemosensitivity and breathing automaticity
Grantee:Thiago dos Santos Moreira
Support type: Research Projects - Thematic Grants
FAPESP's process: 15/12827-2 - Acetylcholine and ventilation: Modulation by cholinergic inputs from pedunculopontine tegmental nucleus to the retrotrapezoid nucleus
Grantee:Cleyton Roberto Sobrinho
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 17/01380-2 - Pedunculo pontine tegmental nucleus and brainstem respiratory interactions in breathing
Grantee:Janayna Dias Lima
Support type: Scholarships in Brazil - Doctorate (Direct)