Crude extract of cyanobacterium Radiocystis fernandoi strain R28 induces anemia and oxidative stress in fish erythrocytes

Author(s)

Sakuragui, Marise Margareth ^{[1, 2]} ; Paulino, Marcelo Gustavo ^{[1, 3]} ; da Silva e Souza, Naiara Elisabete ^[1] ; Tavares, Driele ^[1] ; Terezan, Ana Paula ^[4] ; Pesenti, Emanuele ^[5] ; Giani, Alessandra ^[6] ; Fernandes, Joao Batista ^[4] ; Cestari, Marta Margarete ^[5] ; Fernandes, Marisa Narciso ^[1]

Affiliation

^[1] Univ Fed Sao Carlos, Dept Ciencias Fisiol, Rodovia Washington Luiz Km 235, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Estadual Minas Gerais, Campus Passos, Av Juca Stockler 1130, BR-37900106 Passos, MG - Brazil

^[3] Univ Fed Tocantins, Setor Cimba, Campus Araguaina, Av Paraguai S-N, BR-77824838 Araguaina, TO - Brazil

^[4] Univ Fed Sao Carlos, Dept Quim, Rodovia Washington Luiz Km 235, BR-13565905 Sao Carlos, SP - Brazil

^[5] Univ Fed Parana, Dept Genet, POB 19071, BR-81531990 Curitiba, Parana - Brazil

^[6] Univ Fed Minas Gerais, Dept Bot, Av Pres Antonio Carlos 6627, BR-31270901 Belo Horizonte, MG - Brazil

• Document type: Journal article
• Source: Toxicon; v. 169, p. 18-24, NOV 2019.
• Web of Science Citations: 0

Abstract

The cyanobacterium Radiocystis fernandoi has been frequently identified in cyanobacterial blooms in Brazil. Recently, R. fernandoi strain R28, which produces microcystin (MC)-RR and MC-YR, was isolated from the Furnas reservoir, Minas Gerais, Brazil. The present study evaluated the hematological variables and erythrocyte antioxidant responses, lipid peroxidation (LPG), and genotoxicity in a neotropical fish (Hoplias malabaricus) after acute and subchronic exposure to a crude extract (CE) of R. fernandoi strain R28. Acute exposure (12 or 96 h) consisted of a single intraperitoneal (i.p.) CE injection, and subchronic exposure consisted of one i.p. CE injection every 72 h for 30 days. After acute exposure, fish exhibited macrocytic anemia (12 h post-injection) followed by normocytic anemia (96 h post-injection). The increased activity of superoxide dismutase, glutathione peroxidase, glutathione-S-transferase, and the glutathione level in the erythrocytes did not prevent oxidative stress, manifested as lipid peroxidation and elevated DNA damage after acute exposure. After subchronic exposure, the hematological variables recovered, and the absence of erythrocyte oxidative stress suggests possible modulation by other biological factors, including a possible decrease in MC uptake by the cells and/or increasing detoxification efficiency that precludes erythrocyte damage. (AU)

• FAPESP's process: 12/00728-1 - Effects of microcystins present in the crude extract of cyanobacterium, Microcystis aeruginosa, in the liver and muscle of traírão, Hoplias lacerdae.
• Grantee: Marcelo Gustavo Paulino
• Support Opportunities: Scholarships in Brazil - Doctorate