Solvent and HEMA Increase Adhesive Toxicity and Cytokine Release from Dental Pulp Cells

Author(s)

Massaro, Helder ^[1] ; Zambelli, Ligia F. A. ^[2] ; de Britto, Aurileia A. ^[3] ; Vieira, Rodolfo P. ^{[4, 5, 6, 7]} ; Ligeiro-de-Oliveira, Ana P. ^[3] ; Andia, Denise C. ^[8] ; Oliveira, Marcelo T. ^[9] ; Lima, Adriano F. ^{[8, 10]}

Affiliation

^[1] Univ Paulista, Dept Endodont, Rua Doutor Bacelar 1212, BR-04026002 Sao Paulo - Brazil

^[2] Univ Nove Julho UNINOVE, Dept Restorat Dent, R-01504001 Sao Paulo - Brazil

^[3] Univ Nove Julho UNINOVE, Post Grad Program Biophoton Appl Hlth Sci, BR-01504001 Sao Paulo - Brazil

^[4] Univ Brasil, Postgrad Program Bioengn & Biomed Engn, Rua Carolina Fonseca 235, BR-08230030 Sao Paulo - Brazil

^[5] Fed Univ Sao Paulo UNIFESP, Dept Sci Human Movement & Rehabil, Avenida Ana Costa 95, BR-11060001 Santos - Brazil

^[6] Brazilian Inst Teaching & Res Pulm & Exercise Imm, Rua Pedro Ernesto 240, BR-12245520 Sao Jose Dos Campos - Brazil

^[7] Anhembi Morumbi Univ, Sch Med, Ave Deputado Benedito Matarazzo 4050, BR-12230002 Sao Jose Dos Campos - Brazil

^[8] Univ Paulista, Dent Res Div, Rua Doutor Bacelar 1212, BR-04026002 Sao Paulo - Brazil

^[9] Univ Ibirapuera, Dept Restorat Dent, Av Interlagos 1329, BR-04661100 Sao Paulo, SP - Brazil

^[10] Univ Paulista, Sch Dent, Dent Res Div, Rua Doutor Bacelar 1212, BR-04026002 Sao Paulo - Brazil

• Document type: Journal article
• Source: MATERIALS; v. 12, n. 17 SEP 2019.
• Web of Science Citations: 1

Abstract

The aim of the present study was to evaluate the effect of the hydroxyethyl-methacrylate (HEMA) concentration and solvent content of dental adhesives on cell viability and cytokine (IL-1b, IL-6, IL-10, TNF-alpha) release by human dental pulp cells (HDPCs). HDPCs were obtained from fresh extracted human third molars. Experimental adhesives were prepared containing different concentrations of HEMA (0%, 10%, and 20%) with and without solvent (ethanol 10%). Cylindrical specimens were immersed on culture medium during 24 h to obtain the extracts. The cells were incubated with extracts (culture medium + components leached from the adhesives) of different adhesives, and cell viability and cytokine release were evaluated after 6 and 24 h of exposure. Adhesives containing HEMA promoted high cell viability reduction after 6 h of exposure; but after 24 h, the results were similar to the ones found among control group cells. These effects on cell viability were prominently increased with the addition of solvent. Although IL-1b release was not affected by exposure to eluates, other cytokines (IL-10, IL-6, TNF-alpha) were modulated by the different experiment conditions, directly influenced by the HEMA concentration and presence of solvent. Higher HEMA concentrations, combined with the presence of solvent, can promote significant reduction on HDPC viability, increasing the release of anti- and pro-inflammatory mediators. (AU)

• FAPESP's process: 13/02928-0 - Cytotoxicity of solvated and non-solvated with different concentrations of 2-hydroxietyl-metacrylate on pulp cells
• Grantee: Lígia França Aires Zambelli
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 12/15165-2 - Role of purinergic signaling and of SOCS-JAK-STAT signaling in the antiinflammatory effects of aerobic training in experimental models of asma and in asthmatic individuals
• Grantee: Rodolfo de Paula Vieira
• Support Opportunities: Research Grants - Young Investigators Grants