Addition of phosphates and chlorhexidine to resin-modified MTA materials

Author(s)

Vitti, Rafael Pino ^[1] ; Pacheco, Rafael Rocha ^[2] ; Nogueira Leal Silva, Emmanuel Joao ^[3] ; Prati, Carlo ^[4] ; Gandolfi, Maria Giovanna ^[5] ; Piva, Evandro ^[6] ; Ogliari, Fabricio Aulo ^[7] ; Zanchi, Cesar Henrique ^[6] ; Coelho Sinhoreti, Mario Alexandre ^[8]

Affiliation

^[1] Univ Taubate, Sch Dent, Dept Prosthodont, Sao Paulo - Brazil

^[2] Univ Detroit Mercy, Sch Dent, Dept Restorat Dent, Detroit, MI 48221 - USA

^[3] Grande Rio Univ, Sch Dent, Dept Endodont, Rio De Janeiro - Brazil

^[4] Univ Bologna, Sch Dent, Dept Biomed & NeuroMotor Sci, Bologna - Italy

^[5] Univ Bologna, Sch Dent, Lab Biomat & Oral Pathol, Bologna - Italy

^[6] Univ Fed Pelotas, Sch Dent, Dept Operat Dent, Pelotas, RS - Brazil

^[7] Univ Fed Pelotas, Sch Mat Engn, Dept Organ Chem, Pelotas, RS - Brazil

^[8] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Restorat Dent, Sao Paulo - Brazil

• Document type: Journal article
• Source: JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS; v. 107, n. 6, p. 2195-2201, AUG 2019.
• Web of Science Citations: 0

Abstract

To evaluate the properties of experimental mineral trioxide aggregate (MTA) resin-modified materials for root-end filling procedures, varying their compositions regarding the addition of hydroxiapatite (HA) or dicalcium phosphate dihydrate, with or without chlorhexidine digluconate. White MTA (Angelus, Londrina, Brazil) was used as a reference material. Degree of conversion (DC) was evaluated by Fourier transformed infrared (FTIr) spectroscopy (n = 5). Flowability (n = 3) and radiopacity (n = 3) were evaluated following ISO 6876:2001 methods. For splitting tensile strength analysis, cylindrical samples (n = 10) were subjected to compressive load using a universal testing machine (Instron Corporation, Norwood, MA). Water sorption and solubility tests were performed according to ISO 4049:2009 methods. Calcium ion release and pH analysis (n = 10) were evaluated using a pH meter (Orion, Watsonville, CA). Cytotoxicity (n = 8) of materials extracts was evaluated as cell viability percentage. Statistical analysis was performed using Kolmogorov-Smirnov for normal distribution and data was subjected to one-way ANOVA and Tukey test (a = 0.05). Addition of chlorhexidine digluconate reduced DC mean values for experimental materials (<50%). White MTA demonstrated lower flowability (5.3 mm) and higher radiopacity (9.8 mm Al), splitting tensile strength (9.1 MPa), solubility (8.2 mu g/mm(3)), calcium ion release (similar to 26.5 ppm), cytotoxicity (55.2%), and pH mean values (10.8), when compared to experimental materials. All groups demonstrated a decrease in calcium release (<85%) and pH (<13%). Formulation containing HA demonstrated similar pH values after 28 days when compared to white MTA. Evaluated experimental resin-modified MTA based materials without chlorhexidine digluconate showed satisfactory results for all physicochemical properties tested and cytotoxicity. (c) 2019 Wiley Periodicals, Inc. (AU)

• FAPESP's process: 12/25034-2 - Development of endodontic sealers based on MTA
• Grantee: Rafael Pino Vitti
• Support Opportunities: Scholarships in Brazil - Post-Doctoral