Continuous separation of cytochrome-c PEGylated conjugates by fast centrifugal partition chromatography

Author(s)

Santos, Joao H. P. M. ^{[1, 2]} ; Ferreira, Ana M. ^[1] ; Almeida, Mafalda R. ^[1] ; Quinteiro, Paula S. G. N. ^[3] ; Dias, V, Ana C. R. ; Coutinho, Joao A. P. ^[1] ; Freire, Mara G. ^[1] ; Rangel-Yagui, Carlota O. ^[2] ; Ventura, Sonia P. M. ^[1]

Affiliation

^[1] Univ Aveiro, Dept Chem, CICECO Aveiro Inst Mat, P-3810193 Aveiro - Portugal

^[2] Univ Sao Paulo, Dept Biochem & Pharmaceut Technol, Av Prof Lineu Prestes 580 Bloco 16, BR-05508000 Sao Paulo, SP - Brazil

^[3] V, Univ Aveiro, Dept Environm & Planning, CESAM Ctr Environm & Marine Studies, P-3810193 Aveiro - Portugal

• Document type: Journal article
• Source: GREEN CHEMISTRY; v. 21, n. 20, p. 5501-5506, OCT 21 2019.
• Web of Science Citations: 1

Abstract

Herein, the effective use of aqueous biphasic systems (ABS) in Fast Centrifugal Partition Chromatography (FCPC) for the purification of PEGylated cytochrome c conjugates is shown. High recoveries (between 88% and 100%) and purities (similar to 100%) were obtained. Both the unreacted cytochrome c and solvents may be recovered and reused, thus allowing the design of a sustainable process in a continuous regime for the isolation of bioconjugates. This process allowed the reduction of the complete E-factor and carbon footprint at circa 100% and 67%, respectively, reinforcing the important environmental contribution of recycling units. (AU)

• FAPESP's process: 16/22065-5 - N-terminal pegylation of proteins and purification by aqueous two-phase systems
• Grantee: Carlota de Oliveira Rangel Yagui
• Support Opportunities: Regular Research Grants