Deciphering the Evolutionary History of Arowana Fishes (Teleostei, Osteoglossiformes, Osteoglossidae): Insight from Comparative Cytogenomics

Author(s):Show less -

Cioffi, Marcelo de Bello ^[1] ; Rab, Petr ^[2] ; Ezaz, Tariq ^[3] ; Carlos Bertollo, Luiz Antonio ^[1] ; Lavoue, Sebastien ^[4] ; de Oliveira, Ezequiel Aguiar ^{[5, 1]} ; Sember, Alexandr ^[2] ; Molina, Wagner Franco ^[6] ; Santos de Souza, Fernando Henrique ^[1] ; Majtanova, Zuzana ^[2] ; Liehr, Thomas ^[7] ; Al-Rikabi, Ahmed Basheer Hamid ^[7] ; Yano, Cassia Fernanda ^[1] ; Viana, Patrik ^[8] ; Feldberg, Eliana ^[8] ; Unmack, Peter ^[3] ; Hatanaka, Terumi ^[1] ; Tanomtong, Alongklod ^[9] ; Perez, Manolo Fernandez ^[1]

Affiliation

^[1] Univ Fed Sao Carlos UFSCar, Dept Genet & Evolucao, BR-13565090 Sao Carlos, SP - Brazil

^[2] Czech Acad Sci, Inst Anim Physiol & Genet, Lab Fish Genet, Libechov 27721 - Czech Republic

^[3] Univ Canberra, Inst Appl Ecol, Canberra, ACT 2617 - Australia

^[4] Univ Sains Malaysia, Sch Biol Sci, George Town 11800, Penang - Malaysia

^[5] Secretaria Estado Educ Mato Grosso SEDUC MT, BR-78049909 Cuiaba, MT - Brazil

^[6] Univ Fed Rio Grande do Norte, Ctr Biociencias, Dept Biol Celular & Genet, BR-59078970 Natal, RN - Brazil

^[7] Univ Hosp Jena, Inst Human Genet, D-07747 Jena - Germany

^[8] Inst Nacl de Pesquisas da Amazonia, Coordenacao Biodiversidade, Lab Genet Anim, BR-69077000 Manaus, Am - Brazil

^[9] KhonKaen Univ, Tox Subst Livestock & Aquat Anim Res Grp, Muang 40002, Khonkaen - Thailand

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 20, n. 17 SEP 1 2019.
• Web of Science Citations: 0

Abstract

Arowanas (Osteoglossinae) are charismatic freshwater fishes with six species and two genera (Osteoglossum and Scleropages) distributed in South America, Asia, and Australia. In an attempt to provide a better assessment of the processes shaping their evolution, we employed a set of cytogenetic and genomic approaches, including i) molecular cytogenetic analyses using C- and CMA(3)/DAPI staining, repetitive DNA mapping, comparative genomic hybridization (CGH), and Zoo-FISH, along with ii) the genotypic analyses of single nucleotide polymorphisms (SNPs) generated by diversity array technology sequencing (DArTseq). We observed diploid chromosome numbers of 2n = 56 and 54 in O. bicirrhosum and O. ferreirai, respectively, and 2n = 50 in S. formosus, while S. jardinii and S. leichardti presented 2n = 48 and 44, respectively. A time-calibrated phylogenetic tree revealed that Osteoglossum and Scleropages divergence occurred approximately 50 million years ago (MYA), at the time of the final separation of Australia and South America (with Antarctica). Asian S. formosus and Australian Scleropages diverged about 35.5 MYA, substantially after the latest terrestrial connection between Australia and Southeast Asia through the Indian plate movement. Our combined data provided a comprehensive perspective of the cytogenomic diversity and evolution of arowana species on a timescale. (AU)

• FAPESP's process: 18/22033-1 - An intercontinental approach for the investigation of chromosome evolution, genetic diversity and biogeography in the order Osteoglossiformes (Teleostei: Osteoglossomorpha). Part III
• Grantee: Marcelo de Bello Cioffi
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/10240-0 - Genetic diversity, biogeography and evolution of Osteoglossiformes fishes (Teleostei, Osteoglossomorpha)
• Grantee: Manolo Fernandez Perez
• Support Opportunities: Scholarships in Brazil - Post-Doctoral