Intracerebral Injection of Streptozotocin to Model Alzheimer Disease in Rats

Author(s)

Moreira-Silva, Daniel ^{[1, 2]} ; Vizin, Robson C. L. ^{[1, 3]} ; Martins, Talita M. S. ^[4] ; Ferreira, Tatiana L. ^[5] ; Almeida, Maria C. ^[6] ; Carrettiero, Daniel C. ^[6]

Affiliation

^[1] Fed Univ ABC, Grad Program Neurosci & Cognit, Sao Bernardo Do Campo - Brazil

^[2] Univ Fed Uberlandia, Inst Biomed Sci, Dept Pharmacol, Uberlandia, MG - Brazil

^[3] St Josephs Hosp, Trauma Res, Thermoregulat & Syst Inflammat Lab FeverLab, Phoenix, AZ - USA

^[4] Fed Univ ABC, Undergrad Program Neurosci, Sao Bernardo Do Campo - Brazil

^[5] Fed Univ ABC, Ctr Math Comp & Cognit, Sao Bernardo Do Campo - Brazil

^[6] Fed Univ ABC, Ctr Nat & Human Sci, Sao Bernardo Do Campo - Brazil

• Document type: Journal article
• Source: BIO-PROTOCOL; v. 9, n. 20 OCT 20 2019.
• Web of Science Citations: 0

Abstract

Animal models have promoted meaningful contribution to science including Alzheimer's disease (AD) research. Several animal models for AD have been used, most of them related to genetic mutations observed in familial AD. However, sporadic form of AD, also named late-onset is the most frequent form of the disease, which is multifactorial, being influenced by genetic, environmental and lifestyle factors. Here, we describe a protocol of an AD-like pathology of the sporadic form using Wistar rats by a single bilateral intracerebroventricular (icy) injection of streptozotocin (STZ, 2 mg/kg). Icy injection of STZ induces brain resistance to insulin and other pathological alterations related to those observed in AD, such as cognitive impairment and accumulation of phosphorylated tau protein and beta-amyloid in the brain. Thus, icy injection of STZ is a useful tool to investigate the pathological mechanisms and the metabolic alterations involved in AD and to propose new therapeutic approaches and neuroprotective drugs. (AU)

• FAPESP's process: 14/14661-1 - Role of cannabinoid system on tau activity and its relation to the cognitive performance in a model of sporadic Alzheimer's Disease in rats
• Grantee: Daniel Moreira Silva
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/12648-6 - Effect of environmental temperature on behavioral parameters in animal model for Alzheimer's Disease
• Grantee: Talita Maria Senra Martins
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 15/23426-9 - Beta-amyloid in Alzheimer's Disease: death or survival? Involvement of NF-kappaB and BAG2
• Grantee: Daniel Carneiro Carrettiero
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/24773-7 - Possible preventive effect of anandamide on alterations of neuroplasticity and modulation of endocannabinoid system induced by streptozotocin
• Grantee: Daniel Moreira Silva
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 15/02991-0 - Involvement of TRPM8 channels in thermoregulation of Wistar rats
• Grantee: Maria Camila Almeida
• Support Opportunities: Regular Research Grants