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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The Absence of Pineal Melatonin Abolishes the Daily Rhythm of Tph1 (Tryptophan Hydroxylase 1), Asmt (Acetylserotonin O-Methyltransferase), and Aanat (Aralkylamine N-Acetyltransferase) mRNA Expressions in Rat Testes

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Author(s):
Coelho, L. A. [1] ; Andrade-Silva, J. [2] ; Motta-Teixeira, L. C. [2] ; Amaral, F. G. [3] ; Reiter, R. J. [4] ; Cipolla-Neto, J. [2]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Fac Anim Sci & Food Engn FZEA, Dept Vet Med, Av Duque Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci ICB, Dept Physiol & Biophys, Sao Paulo, SP - Brazil
[3] Fed Univ Sao Paulo UNIFESP, Dept Physiol, Sao Paulo, SP - Brazil
[4] Univ Texas Hlth Sci Ctr San Antonio, Dept Cellular & Struct Biol, San Antonio, TX 78229 - USA
Total Affiliations: 4
Document type: Journal article
Source: Molecular Neurobiology; v. 56, n. 11, p. 7800-7809, NOV 2019.
Web of Science Citations: 0
Abstract

This study examined the effects of pinealectomy in Wistar rats and melatonin replacement therapy on the daily mRNA expression of melatonin (Tph1, Aanat, Asmt, Mt1, Mt2, and Ror alpha), and steroidogenic (Star, 17 beta hsd3, and Lhr) related genes as well as clock genes (Rev-erb alpha, Bmal1, Per1, Per2, Cry1, and Cry2) in testes. The testes of control animals express the Tph1, Aanat, and Asmt and Per2 genes with 24-h rhythms in mRNA, reaching the maximal values during the dark phase. Pinealectomy abolished and melatonin treatment restored the 24-h rhythmicity. Daytime differences in mRNA expression were significant for Star, Lhr, Mt1, Mt2, Ror alpha, Rev-erb alpha, Bmal1, Cry1, and Cry2 genes in testes of control rats. Conversely, 17 beta hsd3 and Per1 mRNA expression did not show a daytime difference in testes of control animals. Pinealectomy abolished the peak time of Mt1 and Mt2 mRNA expression, phase shifted the peak time of Star, Ror alpha, Rev-erb alpha, Bmal1, and Cry2 mRNA expression, downregulated the 24-h Lhr mRNA expression, and inverted the peak time of Per1, Per2, and Cry1 mRNA expression to the light phase. The melatonin replacement therapy completely restored the control levels of Lhr, Rev-erb alpha, and Per1 mRNA expression patterns, partially restored the daily control of Star, Mt2, Ror alpha, Bmal1, Cry1, and Cry2 mRNA expression but did not re-establish the daily control of Mt1 mRNA expression. This suggests that the daily mRNA expression of these genes is probably driven by pineal melatonin and melatonin treatment restores (partially or completely) the daily control of gene expression patterns. (AU)

FAPESP's process: 14/50457-0 - Melatonin and the control of energy metabolism: basic, clinical and epidemiological research
Grantee:José Cipolla Neto
Support type: Research Projects - Thematic Grants
FAPESP's process: 14/17830-9 - The role of melatonin in some aspects of in vitro maturation of mammalian oocytes
Grantee:Lia de Alencar Coelho
Support type: Regular Research Grants