Optimization of limonene biotransformation to limonene-1,2-diol by Colletotrichum nymphaeae CBMAI 0864

Author(s)

Sales, Adones ^[1] ; Pastore, Glaucia Maria ^[1] ; Bicas, Juliano Lemos ^[1]

Affiliation

^[1] Univ Estadual Campinas, Fac Food Engn, Dept Food Sci, Monteiro Lobato St 80, BR-13083862 Campinas, SP - Brazil

• Document type: Journal article
• Source: Process Biochemistry; v. 86, p. 25-31, NOV 2019.
• Web of Science Citations: 1

Abstract

This study aimed to optimize the conditions of the biotransformation of limonene to limonene-1,2-diol by C. nymphaeae. In the initial modeling, in shake flasks, the use of 13.2 g.L-1 biomass, 27 degrees C, 250 rpm, and pH of 6.0 could maximize the production of limonene-1,2-diol (6.75 g.L-1). Subsequently, optimal conditions were transposed to a bioreactor, where agitation and aeration were also evaluated. A minimum 60% of dissolved oxygen was defined for this process. The limonene volatilization could be reduced almost three times when a larger condenser is used. Finally, when the bioreactor was operated at 27 degrees C, 300 rpm, 1 vvm, and with 13.2 g.L-1 biomass, limonene-1,2-diol production reached 7.1, 7.8, and 5.6 g.L-1 after 72 h when using 20 h.L-1 of R-( +)-, S-( - )-limonene or citrus terpene as substrate, respectively. This is approximately the same maximum product concentration found for the shake flasks, but it represents an almost three times higher productivity. (AU)

• FAPESP's process: 16/21619-7 - Evaluation of the biological properties of limonene biotransformation products
• Grantee: Juliano Lemos Bicas
• Support Opportunities: Regular Research Grants