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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Topical and intradermal delivery of PpIX precursors for photodynamic therapy with intense pulsed light on porcine skin model

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Author(s):
Requena, Michelle Barreto [1] ; Vollet Filho, Jose Dirceu [1, 2] ; de Nardi, Andrigo Barboza [3] ; Escobar, Andre [3] ; Adams, Rozana da Rocha [3] ; Bagnato, Vanderlei Salvador [1] ; Campos de Menezes, Priscila Fernanda [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Sao Carlos Inst Phys, Sao Carlos, SP - Brazil
[2] Sao Paulo State Univ UNESP, Inst Geosci & Exact Sci, Rio Claro, SP - Brazil
[3] Sao Paulo State Univ UNESP, Coll Agr & Vet Sci, Jaboticabal, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Lasers in Medical Science; v. 34, n. 9, p. 1781-1790, DEC 2019.
Web of Science Citations: 0
Abstract

In order to purposely decrease the time of the photodynamic therapy (PDT) sessions, this study evaluated the effects of PDT using topical and intradermal delivery of two protoporphyrin (PpIX) precursors with intense pulsed light (IPL) as irradiation source. This study was performed on porcine skin model, using an IPL commercial device (Intense Pulse Light, HKS801). IPL effect on different administration methods of two PpIX precursors (ALA and MAL) was investigated: a topical cream application and an intradermal application using a needle-free, high-pressure injection system. Fluorescence investigation showed that PpIX distribution by needle-free injection was more homogeneous than that by cream, suggesting that a shorter drug-light interval in PDT protocols is possible. The damage induced by IPL-PDT assessed by histological analysis mostly shows modifications in collagens fibers and inflammation signals, both expected for PDT. This study suggested an alternative protocol for the PDT treatment, possibility half of the incubation time and with just 3 min of irradiation, making the IPL-PDT, even more, promising for the clinical treatment. (AU)

FAPESP's process: 09/54035-4 - Facility for advanced studies of biosystems and nanostructured materials
Grantee:Igor Polikarpov
Support Opportunities: Multi-user Equipment Program
FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
Grantee:Vanderlei Salvador Bagnato
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC