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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Peptide-Based Assemblies on Electrospun Polyamide-6/Chitosan Nanofibers for Detecting Visceral Leishmaniasis Antibodies

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Author(s):
Liberato, Michelle S. [1] ; Mancini, Rodrigo S. N. [1] ; Factori, Irina M. [1] ; Ferreira, Fabio F. [1] ; de Oliveira, Vivian L. [2] ; Carnielli, Juliana B. T. [2] ; Guha, Suchismita [3] ; Peroni, Luis A. [4] ; Oliveira, Marcone A. L. [5] ; Alves, Wendel A. [1]
Total Authors: 10
Affiliation:
[1] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210580 Santo Andre, SP - Brazil
[2] Univ Fed Espirito Santo, Nucleo Doencas Infecciosas, BR-29047100 Vitoria, ES - Brazil
[3] Univ Missouri, Dept Phys & Astron, Columbia, MO 65211 - USA
[4] Rheabiotech Co, BR-13084791 Campinas, SP - Brazil
[5] Univ Fed Juiz de Fora, Dept Quim, BR-36036330 Juiz De Fora, MG - Brazil
Total Affiliations: 5
Document type: Journal article
Source: ACS APPLIED ELECTRONIC MATERIALS; v. 1, n. 10, p. 2086-2095, OCT 2019.
Web of Science Citations: 0
Abstract

An alternative label-free electrochemical immunosensor for the rapid detection of Leishmania braziliensis was developed by immobilizing a peptide-based probe of the promastigote surface antigen (PSA-38S) onto electrospun polyamide-6 (PA6)/chitosan nanofibers. An increase in chitosan content in the spinning solution leads to a decrease in the diameter of the formed fibers, whereas the differential scanning calorimetry (DSC) and X-ray diffraction (XRD) data showed a decrease in crystallinity upon increasing the chitosan content in the formulation. In addition, the incorporation of chitosan into the PA6 nanofibers tends to decrease the resistance to the charge-transfer process at the electrode surface. A 40 wt % chitosan content was used for immobilization of the peptide antigen, which was characterized by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). The detection was performed by measuring the relative change in impedance before and after the anti-Leishmania braziliensis reaction by EIS. Under the optimized conditions, the relative change in impedance was proportional to the logarithmic value of PSA concentrations in the range of 2.5 to 10 pg.mL(-1)(r(2) = 0.9946) with a detection limit of 0.2 pg.mL(-1), which was slightly lower than that of the enzyme-linked immunosorbent assay. Additionally, the sensor was tested against two nonspecific antibodies (T. cruzi and beta-actin), whereas multivariate analysis using unsupervised pattern recognition through principal component analysis was successfully applied to identification of the pattern relative to each antibody. Finally, the optimized electrochemical immunoassay can be a favorable approach for Leishmania detection tests, as it is able to differentiate negative and positive visceral leishmaniasis human serum samples. (AU)

FAPESP's process: 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis
Grantee:Marco Aurelio Zezzi Arruda
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 17/02317-2 - Interfaces in materials: electronic, magnetic, structural and transport properties
Grantee:Adalberto Fazzio
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 16/50188-4 - Fabrication and characterization of peptide-polymer hybrid hydrogels for bioanalytical applications
Grantee:Wendel Andrade Alves
Support Opportunities: Regular Research Grants