Study of Leishmania infantum antigen immobilization on organized platforms using Surface Plasmon Resonance and Quartz Crystal Microbalance for the detection of Visceral Leishmaniasis-specific antibodies
This project aims to develop immunosensors based on Surface Plasmon Resonance (SPR) and Quartz Crystal Microbalance (QCM) techniques for the real-time detection of anti-Leishmania infantum antibodies. Extensive studies will be performed for the immobilization of different Leishmania infantum antigenic forms, including crude antigens, recombinant antigens and synthetic peptides on the transducer surface. Self-assembled monolayers (SAMs) conjugated to multivalent systems, as the dendrimers with a number of generations, as well as gold nanoparticles, will be used in combination to improve the control of the surface properties. Thus, these immobilization methods aim at the formation of a highly organized platform, enabling greater immobilization of antigenic molecules in addition to electrical and/or optical signal amplification. The characterization of these immobilization platforms will be carried out by Cyclic Voltammetry (CV), Differential Pulse Voltammetry (DPV), Electrochemical Impedance Spectroscopy (EIS), Scanning Electrochemical Microscopy (SECM), and Scanning Electron Microscopy (SEM) techniques. Afterward, the antigens will be added on the molecular organization and the immobilization monitored in real time by SPR and QCM. VC, EIS and SECM techniques will be used in the immunosensor characterization. The sensitivity and selectivity of the sensor will be evaluated by adding to its surface of canine sera which are positive and negative for Visceral Leishmaniasis (VL). Finally, the results will be compared with conventional methods for VL detection.
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