Study of Leishmania infantum antigen immobilization on organized platforms using Surface Plasmon Resonance and Quartz Crystal Microbalance for the detection of Visceral Leishmaniasis-specific antibodies

• Grant number: 12/24493-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): March 01, 2013
• Effective date (End): May 31, 2016
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Lauro Tatsuo Kubota
• Grantee: Dênio Emanuel Pires Souto
• Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

This project aims to develop immunosensors based on Surface Plasmon Resonance (SPR) and Quartz Crystal Microbalance (QCM) techniques for the real-time detection of anti-Leishmania infantum antibodies. Extensive studies will be performed for the immobilization of different Leishmania infantum antigenic forms, including crude antigens, recombinant antigens and synthetic peptides on the transducer surface. Self-assembled monolayers (SAMs) conjugated to multivalent systems, as the dendrimers with a number of generations, as well as gold nanoparticles, will be used in combination to improve the control of the surface properties. Thus, these immobilization methods aim at the formation of a highly organized platform, enabling greater immobilization of antigenic molecules in addition to electrical and/or optical signal amplification. The characterization of these immobilization platforms will be carried out by Cyclic Voltammetry (CV), Differential Pulse Voltammetry (DPV), Electrochemical Impedance Spectroscopy (EIS), Scanning Electrochemical Microscopy (SECM), and Scanning Electron Microscopy (SEM) techniques. Afterward, the antigens will be added on the molecular organization and the immobilization monitored in real time by SPR and QCM. VC, EIS and SECM techniques will be used in the immunosensor characterization. The sensitivity and selectivity of the sensor will be evaluated by adding to its surface of canine sera which are positive and negative for Visceral Leishmaniasis (VL). Finally, the results will be compared with conventional methods for VL detection.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SOUTO, DENIO E. P.; FONSECA, ALIANI M.; BARRAGAN, JOSE T. C.; LUZ, RITA DE C. S.; ANDRADE, HELIDA M.; DAMOS, FLAVIO S.; KUBOTA, LAURO T.. SPR analysis of the interaction between a recombinant protein of unknown function in Leishmania infantum immobilised on dendrimers and antibodies of the visceral leishmaniasis: A potential use in immunodiagnosis. BIOSENSORS & BIOELECTRONICS, v. 70, p. 275-281, AUG 15 2015. (12/24493-3)

SOUTO, DENIO E. P.; FARIA, ANGELICA R.; DE ANDRADE, HELIDA M.; KUBOTA, LAURO T.. Using QCM and SPR for the Kinetic Evaluation of the Binding Between A New Recombinant Chimeric Protein and Specific Antibodies of the Visceral Leishmaniasis. CURRENT PROTEIN & PEPTIDE SCIENCE, v. 16, n. 8, p. 782-790, 2015. (12/24493-3)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SOUTO, Dênio Emanuel Pires. Study of the immobilization of Leishmania infantum antigens on organized platforms using SPR and QCM for the detection of specifics antibodies of the Visceral Leishmaniasis. 2016. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Química Campinas, SP.