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Development of microgravimetric biosensors by means of self-assembled monolayers


In the present project we propose to develop microgravimetric biosensor using lectins as active material immobilized by self-assembled monolayers technique on gold electrode of quartz crystal. Therefore, the quartz crystal microbalance will be used as transducer for monitoring the frequency variation of the crystal. The immobilization of the protein by means of self-assembled monolayers will be monitored by using in situ mass variation, infrared reflection absorption spectroscopy and electroacoustic impedance in partnership with Dr. Paulo R. Bueno, (IQ-UNESP-Araraquara). As active biomaterial it will be used lectins obtained from jackfruit seeds (artocarpus integrifolia) supplied by Dra. Maria Cristina Roque Barreira (FM-USP-RP). The chemical interaction of the lectin with different monosaccharide is intended to be study with the main goal of quantify which carbohydrate possess the larger affinity with lectin. This is a methodology to determine the affinity constants between lectin and carbohydrate. Once the lectins possess high affinity for different glycoconjugates, studies will be conducted to determine the biosensor response of such analytes, regarding sensibility, selectivity, detection limit and biosensor lifetime. (AU)

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(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PEDROSO‚ M.M.; WATANABE‚ A.M.; ROQUE-BARREIRA‚ M.C.; BUENO‚ P.R.; FARIA‚ R.C. Quartz Crystal Microbalance monitoring the real-time binding of lectin with carbohydrate with high and low molecular mass. Microchemical Journal, v. 89, n. 2, p. 153-158, 2008.

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