| Full text | |
| Author(s): |
Lukassen, Mie B.
[1]
;
de Jonge, Nadieh
[1]
;
Bjerregaard, Sabine M.
[1]
;
Podduturi, Raju
[2]
;
Jorgensen, Niels O. G.
[2]
;
Petersen, Mikael A.
[3]
;
David, Gianmarco S.
[4]
;
da Silva, Reinaldo J.
[5]
;
Nielsen, Jeppe L.
[1]
Total Authors: 9
|
| Affiliation: | [1] Aalborg Univ, Dept Chem & Biosci, Aalborg - Denmark
[2] Univ Copenhagen, Dept Plant & Environm Sci, Copenhagen - Denmark
[3] Univ Copenhagen, Dept Food Sci, Sect Design & Consumer Behav, Copenhagen - Denmark
[4] Sao Paulo State Agribusiness Technol Jau, Jau - Brazil
[5] Univ Estadual Paulista Julio Mesquite Filho, Inst Biociencias, Dept Parasitol, Botucatu, SP - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | FRONTIERS IN MICROBIOLOGY; v. 10, OCT 31 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
Aim To determine major sources of microbially produced geosmin in the commercially important aquaculture fish species tilapia. Methods and Results Abundance and composition of geosmin-producing bacteria in water and fish biosphere (intestine, digesta, and fins) of Nile tilapia (Oreachromis niloticus) raised in net cages in Brazilian freshwater farms were examined. By combining qPCR of the geosmin synthase geoA gene and 16S rRNA gene amplicon sequencing to identify potential geosmin-producing organisms, we observed that the proportion and composition of geosmin producers appeared to be rather similar in the water, digesta, intestinal mucous, and on skin, making up about 0.1-0.2% of the total bacterial densities. A high proportion of Cyanobacteria and other putative geosmin producers affiliated to the Actinomycetales were identified in the intestinal mucous layer. The main uptake site for geosmin in fish is traditionally assumed to be through the gill surface, but the present results suggest that uptake by the intestinal tract may represent a major source of geosmin uptake in fish. Conclusion The high abundance of geosmin-producing bacteria in the intestinal mucous layer and digesta may indicate that the digestive system in fish is an important, but hitherto overlooked, source of geosmin and likely other off-flavors in fish. (AU) | |
| FAPESP's process: | 13/50504-5 - Improved quality of cultured fish for human consumption |
| Grantee: | Reinaldo José da Silva |
| Support Opportunities: | Research Projects - Thematic Grants |