| Full text | |
| Author(s): Show less - |
Fonseca, Felipe Paiva
[1, 2]
;
Soares Macedo, Carolina Carneiro
[1]
;
dos Santos Costa, Sara Ferreira
[2]
;
Paes Leme, Adriana Franco
[3]
;
Rodrigues, Romenia Ramos
[3]
;
Rebelo Pontes, Helder Antonio
[4]
;
Altemani, Albina
[5]
;
van Heerden, Willie F. P.
[6]
;
Martins, Manoela Domingues
[7, 1]
;
de Almeida, Oslei Paes
[1]
;
Santos-Silva, Alan Roger
[1]
;
Lopes, Marcio Ajudarte
[1]
;
Vargas, Pablo Agustin
[1, 6]
Total Authors: 13
|
| Affiliation: | [1] Univ Campinas UNICAMP, Piracicaba Dent Sch, Oral Pathol Div, Dept Oral Diag, Piracicaba - Brazil
[2] Univ Fed Minas Gerais, Sch Dent, Dept Surg & Pathol, Belo Horizonte, MG - Brazil
[3] CNPEM, Lab Nacl Biociencias LNBio, Lab Espectrometria Massas, Campinas, SP - Brazil
[4] Fed Univ Para, Serv Oral Pathol, Joao de Barros Barreto Univ Hosp, Belem, Para - Brazil
[5] Univ Campinas UNICAMP, Sch Med Sci, Dept Pathol, Campinas, SP - Brazil
[6] Univ Pretoria, Sch Dent, Dept Oral Pathol & Oral Biol, Pretoria - South Africa
[7] Univ Fed Rio Grande do Sul, Sch Dent, Dept Pathol, Porto Alegre, RS - Brazil
Total Affiliations: 7
|
| Document type: | Journal article |
| Source: | ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY; v. 128, n. 6, p. 639-650, DEC 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
Objective. The aim of this study was to determine the proteome of adenoid cystic carcinoma (AdCC) and polymorphous adenocarcinoma (PAc) and to identify a protein signature useful in distinguishing these two neoplasms. Study Design. Ten cases of AdCC and 10 cases of PAc were microdissected for enrichment of neoplastic tissue. The samples were submitted to liquid chromatography-tandem mass spectrometry (LC-MS/MS), and the proteomics data were analyzed by using the MaxQuant software. LC-MS/MS spectra were searched against the Human UniProt database, and statistical analyses were performed with Perseus software. Bioinformatic analyses were performed by using discovery-based proteomic data on both tumors. Results. LC-MS/MS analysis identified 1957 proteins. The tumors shared 1590 proteins, and 261 were exclusively identified in AdCC and 106 in PAc. Clustering analysis of the statistically significant proteins clearly separated AdCC from PAc. Protein expression 10 times higher in one group than in the other led to a signature of 16 proteins-6 upregulated in AdCC and 10 in PAc. A new clustering analysis showed reverse regulation and also differentiated both tumors. Conclusions. Global proteomics may be useful in discriminating these two malignant salivary neoplasms that frequently show clinical and microscopic overlaps, but additional validation studies are still necessary to determine the diagnostic potential of the protein signature obtained. (AU) | |
| FAPESP's process: | 09/54067-3 - Acquisition of a mass spectrometer coupled to a liquid chromatography system for increasing the capacity to meet the needs of users and for making new technologies available in the Laboratory of Mass Spectrometry |
| Grantee: | Adriana Franco Paes Leme |
| Support Opportunities: | Multi-user Equipment Program |
| FAPESP's process: | 15/16056-0 - ADENOCARCINOMA NOT OTHERWISE SPECIFIED OF SALIVARY GLANDS: HISTOPATHOLOGICAL, PROTEIC AND GENETIC STUDY |
| Grantee: | Felipe Paiva Fonseca |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |