Neonatal anoxia impairs long-term energy metabolism and somatic development of Wistar rats

Author(s)

Cruz-Ochoa, Natalia Andrea ^[1] ; Esperanza Ochoa-Amaya, Julieta ^[2] ; Lorena Pulecio, Sandy ^[2] ; Xavier, Gilberto Fernando ^[3] ; Nogueira, Maria Ines ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Anat, Lab Neurociencias, Ave Prof Lineu Prestes 2415, BR-05508900 Sao Paulo, SP - Brazil

^[2] Univ Llanos, Fac Ciencias Agr & Recursos Nat, Programa Med Vet & Zootecnia, Km 12 Via Puerto Lopez, Villavicencio, Meta - Colombia

^[3] Univ Sao Paulo, Inst Biociencias, Dept Fisiol, Rua Matao, Travessa 14, 101, BR-05508900 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE; v. 79, p. 76-85, DEC 2019.
• Web of Science Citations: 0

Abstract

Background: Neonatal anoxia may cause neurological injuries, behavioral alterations and changes in somatic growth. Somatic developmental changes suggest a possible effect of anoxia on energy metabolism and/or feeding behavior. Short-term effects of oxygen deficit on energy homeostasis have been described. In contrast, just a few studies report long-term effects. This study investigated the effects of neonatal anoxia on energy metabolism and somatic development at adulthood of males and females Wistar rats. Method: Male (m) and female (f) rats were exposed, on postnatal day 2 (P2), to either 25-min of Anoxia or Control treatment. At P34 part of the subjects of each group was fasted for 18 h, refeed for 1 h and then perfused 30 min later, at P35; the remaining subjects were submitted to these treatments at P94 and perfused at P95. Therefore, there were 8 groups: AmP35, AmP95, AfP35, AfP95, CmP35, CmP95, CfP35 and CfP95. For subjects perfused at P95, body weight and food intake were recorded up to P90. For subjects perfused at P35 and P95, glycemia, leptin and insulin were assessed after fasting and refeed. After perfusion the encephalon and pancreas were collected for Fos immunohistochemistry and Hematoxylin-Eosin stain analyses. Results: Even though neonatal anoxia did not interfere with regular food intake, it reduced body weight gain along growing in both male and female subjects as compared to the corresponding controls. At P35 neonatal anoxia decreased post-prandial glycemia and increased insulin. While at P95 neonatal anoxia altered the pancreatic histomorphology and increased post-fasting weight loss, decreasing leptin, insulin and glycemia secretion, as well Fos immunoreactivity (IR) in ARC. Conclusion: Neonatal anoxia impairs long-term energy metabolism and somatic development in Wistar rats, with differences related to sex and age. (AU)

• FAPESP's process: 15/18415-8 - Gender differences in ontogenetic development, behavior and energy metabolism in rats subjected to neonatal anoxia: focus on the hippocampus, hypothalamus, leptin and glia cels
• Grantee: Maria Inês Nogueira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/52068-0 - The putative envolvement of melanin-concentrating hormone in the control of lactation
• Grantee: Jackson Cioni Bittencourt
• Support Opportunities: Research Projects - Thematic Grants