The putative envolvement of melanin-concentrating hormone in the control of lactation

• Grant number: 10/52068-0
• Support Opportunities: Research Projects - Thematic Grants
• Field of knowledge: Biological Sciences - Morphology
• Principal Investigator: Jackson Cioni Bittencourt
• Grantee: Jackson Cioni Bittencourt
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Pesquisadores principais: Luciano Freitas Felicio ; Newton Sabino Canteras
• Associated scholarship(s): 13/03694-3 - Action of the melanin-concentrating hormone on neurons of the medial preoptic area in the lactation phase of Sprague-Dawley rats, BP.IC; 12/22122-8 - Study of efferent connections of the ventral and dorsal part of the medial preoptic area, BP.IC

Abstract

Twenty years have passed since the neuropeptide known as melanin-concentrating hormone (MCH) was discovered in mammals. During that time, much has been done in order to elucidate the functions in which MCH is involved. Studies have shown that MCH can function as an orexigenic peptide, as well as playing a role in the control of sensory information, in the reward from food intake, in learning, in memory and in many other functions. In the rat, the prepro-MCH (ppMCH) mRNA and the MCH and NEI peptides are primarily located in the neurons of two central nervous system (CNS) regions: the lateral hypothalamic area and the incerto-hypothalamic area. The axons of those neurons are distributed along the length of the neural axis, including here the median eminence and the posterior lobe of the hypophyisis. However, novel sites of prepro-MCH expression and immunoreactivity (MCH-ir) have been identified in other hypothalamic nuclei, such as the ventral part of medial preoptic area (MPOAv), where such expression has been shown to occur during the final portion of the lactation period (from day 19 to day 21). Nevertheless, very little attention has been given to increase the understanding of the role that MCH plays in maternal behavior (MB). Goal: The objective of this project is to generate additional data on the participation of MCH in the control of lactation, thereby increasing the knowledge regarding the control of MB. Rationale: In lactating rat dams, prepro-MCH and its neuropeptides appear In the MPOAv, specifically near the end of the lactation period, besides the previously mentioned regions, i.e, the LHA and IHy. Therefore, there is a need to improve understanding of the hormonal/neural controls for the termination/continuation of lactation. Material and Methods: In order to study the possible involvement of MCH in MB, we will use transmission electron microscopy of the MCH-ir axons/terminals that traverse the median eminence (internaI lamina), determining their relationship with oxytocinergic axons/terminals, as well as of those found in the posterior lobe of the hypophysis, and it will be analyzed the ultrastructure of the MCH-ir neurons in the MPOAv; we will cannulate the MPOAv and inject it with interference RNA, which will allow us to study the termination/continuation of lactation; using Phaseolus vulgaris leucoagglutinin as an anterograde neuronal tracer, we will also study the efferents of the MPOAv, looking at the targeted areas in order to infer the possible MB-related functions of this specific subdivision; in addition, we will analyze the serum levels of MCH, prolactin, oxytocin and other hormones/neurotransmitters related to lactation, in order to evaluate the relationship between each of those substances and this aspect of MB; we will also use microarray technology in order to study the distribution of the subtypes of the glutamate receptor N-methyl-d-aspartate in MCHergic neurons during the lactation phase. (AU)

Scientific publications

(17)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ORECHIO, DAILIANY; AGUIAR, BRUNA ANDRADE; DINIZ, GIOVANNE BARONI; BITTENCOURT, JACKSON CIONI; HAEMMERLE, CARLOS A. S.; WATANABE, II-SEI; MIGLINO, MARIA ANGELICA; CASTELUCCI, PATRICIA. Morphological and Cellular Characterization of the Fetal Canine (Canis lupus familiaris) Subventricular Zone, Rostral Migratory Stream, and Olfactory Bulb. Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology, v. 301, n. 9, SI, p. 1570-1584, SEP 2018. (16/02224-1, 16/02748-0, 14/25927-2, 10/52068-0)

DINIZ, GIOVANNE B.; CANDIDO, PAULO L.; KLEIN, MARIANNE O.; ALVISI, RENATO D.; PRESSE, FRANCOISE; NAHON, JEAN-LOUIS; FELICIO, LUCIANO F.; BITTENCOURT, JACKSON C.. The weaning period promotes alterations in the orexin neuronal population of rats in a suckling-dependent manner. Brain Structure & Function, v. 223, n. 8, p. 3739-3755, NOV 2018. (17/17998-5, 16/02224-1, 16/02748-0, 10/52068-0)

PEREIRA, LAIS DA SILVA; GOBBO, DENISE RIBEIRO; FERREIRA, JOZELIA GOMES PACHECO; HORTA-JUNIOR, JOSE DE ANCHIETA DE CASTRO E; SA, SUSANA ISABEL; BITTENCOURT, JACKSON CIONI. Effects of ovariectomy on inputs from the medial preoptic area to the ventromedial nucleus of the hypothalamus of young adult rats. Journal of Anatomy, v. 238, n. 2, SEP 2020. (16/02224-1, 10/52068-0, 16/13136-6)

MALTA, MARILIA B.; MARTINS, JOELCIMAR; NOVAES, LEONARDO S.; DOS SANTOS, NILTON B.; SITA, LUCIANE; CAMARINI, ROSANA; SCAVONE, CRISTOFORO; BITTENCOURT, JACKSON; MUNHOZ, CAROLINA D.. Norepinephrine and Glucocorticoids Modulate Chronic Unpredictable Stress-Induced Increase in the Type 2 CRF and Glucocorticoid Receptors in Brain Structures Related to the HPA Axis Activation. Molecular Neurobiology, v. 58, n. 10, JUL 2021. (12/24002-0, 16/02224-1, 12/24727-4, 10/52068-0, 10/13843-8, 16/03572-3)

TESSARIN, GESTTER W. L.; MICHALEC, OLA M.; TORRES-DA-SILVA, KELLY R.; DA SILVA, V, ANDRE; CRUZ-RIZZOLO, ROELF J.; GONCALVES, ALAIDE; GASPARINI, DANIELE C.; HORTA-JUNIOR, JOSE A. C.; ERVOLINO, EDILSON; BITTENCOURT, JACKSON C.; et al. A Putative Role of Teneurin-2 and Its Related Proteins in Astrocytes. FRONTIERS IN NEUROSCIENCE, v. 13, JUN 27 2019. (09/54141-9, 13/26779-4, 10/52068-0, 12/08833-9, 08/02771-6)

ALVISI, R. D.; DINIZ, G. B.; DA-SILVA, J. M.; BITTENCOURT, J. C.; FELICIO, L. F.. Suckling-induced Fos activation and melanin-concentrating hormone immunoreactivity during late lactation. Life Sciences, v. 148, p. 241-246, MAR 1 2016. (10/52068-0)

GOBBO, DENISE RIBEIRO; PEREIRA, LAIS DA SILVA; PACHECO FERREIRA, JOZELIA GOMES; DE CASTRO HORTA-JUNIOR, JOSE ANCHIETA; BITTENCOURT, JACKSON CIONI; SA, SUSANA ISABEL. Effects of ovariectomy on the inputs from the medial nucleus of the amygdala to the ventromedial nucleus of the hypothalamus in young adult rats. Neuroscience Letters, v. 746, FEB 16 2021. (16/02224-1, 10/52068-0, 15/05990-4)

BATTAGELLO, DANIELLA S.; LORENZON, ALINE R.; DINIZ, GIOVANNE B.; MOTTA-TEIXEIRA, LIVIA C.; KLEIN, MARIANNE O.; FERREIRA, JOZELIA G. P.; ARIAS, CARLOS M.; ADAMANTIDIS, ANTOINE; SITA, V, LUCIANE; CIPOLLA-NETO, JOSE; et al. The Rat Mammary Gland as a Novel Site of Expression of Melanin-Concentrating Hormone Receptor 1 mRNA and Its Protein Immunoreactivity. FRONTIERS IN ENDOCRINOLOGY, v. 11, JUL 31 2020. (15/05990-4, 16/02748-0, 17/16293-8, 10/52068-0, 16/04210-8, 17/17998-5, 14/50457-0, 12/04554-8, 14/13052-1, 16/18941-4, 16/19845-9, 16/02224-1)

ORECHIO, DAILIANY; AGUIAR, BRUNA ANDRADE; DINIZ, GIOVANNE BARONI; BITTENCOURT, JACKSON CIONI; HAEMMERLE, CARLOS A. S.; WATANABE, II-SEI; MIGLINO, MARIA ANGELICA; CASTELUCCI, PATRICIA. Morphological and Cellular Characterization of the Fetal Canine (Canis lupus familiaris) Subventricular Zone, Rostral Migratory Stream, and Olfactory Bulb. Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology, v. 301, n. 9, p. 15-pg., 2018-09-01. (16/02748-0, 16/02224-1, 10/52068-0, 14/25927-2)

KRONKA SOSTHENES, MARCIA CONSENTINO; DINIZ, DANIEL GUERREIRO; ROODSELAAR, JAY; ABADIE-GUEDES, RICARDO; CHAVES DE SIQUEIRA MENDES, FABIOLA DE CARVALHO; FERNANDES, TAIANY NOGUEIRA; BITTENCOURT, JACKSON CIONI; PICANCO DINIZ, CRISTOVAM WANDERLEY; ANTHONY, DANIEL CLIVE; ARAUJO GUEDES, RUBEM CARLOS. Stereological Analysis of Early Gene Expression Using Egr-1 Immunolabeling After Spreading Depression in the Rat Somatosensory Cortex. FRONTIERS IN NEUROSCIENCE, v. 13, SEP 25 2019. (10/52068-0, 16/02224-1)

DINIZ, GIOVANNE B.; BITTENCOURT, JACKSON C.. The Melanin-Concentrating Hormone as an Integrative Peptide Driving Motivated Behaviors. FRONTIERS IN SYSTEMS NEUROSCIENCE, v. 11, MAY 29 2017. (16/02748-0, 10/52068-0)

MOTTA-TEIXEIRA, LIVIA CLEMENTE; MACHADO-NILS, ALINE VILAR; BATTAGELLO, DANIELLA SABINO; DINIZ, GIOVANNE BARONI; ANDRADE-SILVA, JESSICA; SILVA JR, SINESIO; MATOS, RAPHAEL AFONSO; DO AMARAL, FERNANDA GASPAR; XAVIER, GILBERTO FERNANDO; BITTENCOURT, JACKSON CIONI; et al. The absence of maternal pineal melatonin rhythm during pregnancy and lactation impairs offspring physical growth, neurodevelopment, and behavior. Hormones and Behavior, v. 105, p. 146-156, SEP 2018. (12/04554-8, 16/02224-1, 16/02748-0, 10/52068-0, 14/22313-3, 14/50457-0, 16/18941-4)

DINIZ, GIOVANNE B.; BITTENCOURT, JACKSON C.. The Melanin-Concentrating Hormone (MCH) System: A Tale of Two Peptides. FRONTIERS IN NEUROSCIENCE, v. 13, NOV 26 2019. (16/02748-0, 16/02224-1, 10/52068-0)

CRUZ-OCHOA, NATALIA ANDREA; ESPERANZA OCHOA-AMAYA, JULIETA; LORENA PULECIO, SANDY; XAVIER, GILBERTO FERNANDO; NOGUEIRA, MARIA INES. Neonatal anoxia impairs long-term energy metabolism and somatic development of Wistar rats. INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE, v. 79, p. 76-85, DEC 2019. (15/18415-8, 10/52068-0)

PACHECO FERREIRA, JOZELIA GOMES; BITTENCOURT, JACKSON CIONI; ADAMANTIDIS, ANTOINE. Melanin-concentrating hormone and sleep. CURRENT OPINION IN NEUROBIOLOGY, v. 44, p. 152-158, JUN 2017. (16/19845-9, 10/52068-0, 04/13849-5, 15/05990-4)

WEISSMANN, LAIS; QUARESMA, PAULA G. F.; SANTOS, ANDRESSA C.; DE MATOS, ALEXANDRE H. B.; PASCOAL, VINICIUS D'AVILA BITTENCOURT; ZANOTTO, TAMIRES M.; CASTRO, GISELE; GUADAGNINI, DIOZE; DA SILVA, JOELCIMAR MARTINS; VELLOSO, LICIO A.; et al. IKK epsilon Is Key to Induction of Insulin Resistance in the Hypothalamus, and Its Inhibition Reverses Obesity. Diabetes, v. 63, n. 10, p. 3334-3345, OCT 2014. (10/52068-0, 13/07607-8, 12/10338-6)

PACHECO FERREIRA, JOZELIA GOMES; GOMES DUARTE, JESSICA CATHARINE; DINIZ, GIOVANNE BARONI; BITTENCOURT, JACKSON CIONI. Litter size determines the number of melanin-concentrating hormone neurons in the medial preoptic area of Sprague Dawley lactating dams. Physiology & Behavior, v. 181, p. 75-79, NOV 1 2017. (16/02224-1, 16/02748-0, 10/52068-0, 15/05990-4, 16/19845-9)