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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Green Tea Prevents NAFLD by Modulation of miR-34a and miR-194 Expression in a High-Fat Diet Mouse Model

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Author(s):
Torres, L. F. [1] ; Cogliati, B. [2] ; Otton, R. [1]
Total Authors: 3
Affiliation:
[1] Cruzeiro do Sul Univ, Interdisciplinary Postgrad Programme Hlth Sci, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: OXIDATIVE MEDICINE AND CELLULAR LONGEVITY; v. 2019, DEC 4 2019.
Web of Science Citations: 0
Abstract

Background/Aims. Nonalcoholic fatty liver disease (NAFLD) is considered the hepatic manifestation of metabolic syndrome. It is currently the most common chronic liver disease with complex pathogenesis and challenging treatment. Here, we investigated the hepatoprotective role of green tea (GT) and determined the involvement of miRNAs and its mechanism of action. Methods. Male C57Bl/6 mice were fed with a high-fat diet for 4 weeks. After this period, the animals received gavage with GT (500 mg/kg body weight) over 12 weeks (5 days/week). HepG2 cell lines were transfected with miR-34a or miR-194 mimetics and inhibitors to validate the in vivo results or were treated with TNF-alpha to evaluate miRNA regulation. Results. GT supplementation protects against NAFLD development by altering lipid metabolism, increasing gene expression involved in triglycerides and fatty acid catabolism, and decreasing uptake and lipid accumulation. This phenotype was accompanied by miR-34a downregulation and an increase in their mRNA targets Sirt1, Ppar alpha, and Insig2. GT upregulated hepatic miR-194 by inhibiting TNF-alpha action leading to a decrease in miR-194 target genes Hmgcs/Apoa5. Conclusion. Our study identified for the first time that the beneficial effects of GT in the liver can be due to the modulation of miRNAs, opening new perspectives for the treatment of NAFLD focusing on epigenetic regulation of miR-34a and miR-194 as green tea targets. (AU)

FAPESP's process: 13/22293-0 - Identification and validation of miRNA target genes in adipose tissue and liver of obese mice in response to treatment with green tea extract
Grantee:Rosemari Otton
Support type: Regular Research Grants
FAPESP's process: 15/21785-1 - Epigenetic regulation: evaluation of miRNAs as potential therapeutic targets in non-alcoholic fatty liver disease (NAFLD) diet-induced obese mice treated with Camelia sinensis
Grantee:Letícia Torres Ferreira
Support type: Scholarships in Brazil - Master